Mouse Galectin-9 Protein, GST Tag_天津卡梅德科技发展有限公司

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Mouse Galectin-9 Protein, GST Tag

Mouse Galectin-9 Protein, GST Tag

产品编号

KMP2325

别名

半乳糖凝集素, Galectin-9, Lgals9

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Catalog Number	KMP2325
Alias	半乳糖凝集素, Galectin-9, Lgals9
Size	50ug, 100ug, 200ug
Product Description	The Mouse Galectin-9 Protein(KMP2325) is produced in E.coli and the target gene encoding Met1-Thr322 is expressed with a GST tag at the N-terminus.
Molecular Name	Galectin-9
Product Introduction	Galectin-9：β-半乳糖苷结合凝集素，以分泌形式参与免疫细胞活化和功能调控。
Molecular Weight	35.42 kDa
Expression System	E. coli
Species	Mouse
Purity	>90%
SDS-PAGE
Purification	Affinity Purification
Uniprot ID	O08573#O08573-2
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	20mM PB, 1mM EDTA, 5mM p-ME, 5% Trehalose, 400mM NaCl, pH6.5
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	Galectin-9 is a cytoplasmic protein that contains two galectin domains. Galectin-9 is an S-type lectin that is over-expressed in Hodgkin's disease tissue. Galectin-9 binds galactosides and has high affinity for the Forssman pentasaccharide. Galectin-9 plays a role in thymocyte-epithelial interactions relevant to the biology of the thymus and Inhibits cell proliferation. Galectin-9 is a ligand for HAVCR2/TIM3 and induces T-helper type 1 lymphocyte(Th1) death. In addition, Galectin-9 suppresses tumor cell metastasis by interfering with the associations CD44, VCAM-1, Integrin α4β1.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

蛋白溶解后浓度偏低，如何调整？

低速离心：12,000 rpm, 5分钟，去除不溶物。缓冲液置换：使用超滤离心管（如10 kDa截留分子量）浓缩。
如何提高重组蛋白的可溶性表达？

可优化表达条件（如降低诱导温度、调整IPTG浓度）、使用促溶标签（如SUMO、GST）、共表达分子伴侣，或尝试不同宿主系统（如哺乳动物细胞或昆虫细胞）以提高可溶性蛋白产量。

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