Mouse Gi24 Protein, His Tag_天津卡梅德科技发展有限公司

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Mouse Gi24 Protein, His Tag

Mouse Gi24 Protein, His Tag

产品编号

KMP2323

别名

V 集免疫调节受体, Platelet receptor Gi24, Gi24

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Catalog Number	KMP2323
Alias	V 集免疫调节受体, Platelet receptor Gi24, Gi24
Size	50ug, 100ug, 200ug
Product Description	The Mouse Gi24 Protein(KMP2323) is produced in HEK293 Cells and the target gene encoding Phe33-Ala191 is expressed with a 6His tag at the C-terminus.
Molecular Name	Gi24
Product Introduction	Gi24（VSIG4）：免疫球蛋白超家族跨膜蛋白，特异性表达于巨噬细胞，参与免疫耐受的建立。
Molecular Weight	18.96 kDa
Expression System	HEK293 Cells
Species	Mouse
Purity	>95%
SDS-PAGE
Purification	Affinity Purification
Uniprot ID	Q9D659
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	PBS, pH7.4
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	Mouse Platelet receptor Gi24(VISTA) is a transmembrane glycoprotein with homology to B7like immune costimulatory molecules. Mature mouse Gi24 contains a 159 amino acid(aa) extracellular domain(ECD) with one V-type Ig-like domain, a 21 aa transmembrane segment, and a 97 aa cytoplasmic domain. VISTA promotes both MT1-MMP expression and the MT1-MMP mediated activation of MMP-2. It supports the differentiation of embryonic stem cells(ESC) and enhances BMP-4 induced signaling in ESC, but it is also down-regulated following BMP-4 exposure. It binds to BMP-4 directly and also associates with the type I BMP receptor Activin RIB/ALK-4. It is expressed on the surface of na-ve CD4+ T cells and regulatory T cells. It is up-regulated in vivo on activated monocytes and dendritic cells. VISTA inhibits CD4+ and CD8+ T cell proliferation and their production of IL-2 and IFN-γ. Its expression on tumor cells attenuates the antitumor immune response and enables more rapid tumor progression.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

如何提高重组蛋白的可溶性表达？

可优化表达条件（如降低诱导温度、调整IPTG浓度）、使用促溶标签（如SUMO、GST）、共表达分子伴侣，或尝试不同宿主系统（如哺乳动物细胞或昆虫细胞）以提高可溶性蛋白产量。
重组蛋白纯化常用的方法有哪些？

常用的纯化方法包括亲和层析（如His-Tag、GST-Tag）、离子交换层析、疏水相互作用层析（HIC）和凝胶过滤层析。具体方法取决于蛋白特性和标签类型。

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