Human INDO Protein, His Tag_天津卡梅德科技发展有限公司

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Human INDO Protein, His Tag

Human INDO Protein, His Tag

产品编号

KMP2319

别名

吲哚 2, 3-二氧合酶 , Indole 2, 3-dioxygenase, INDO

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Catalog Number	KMP2319
Alias	吲哚 2, 3-二氧合酶 , Indole 2, 3-dioxygenase, INDO
Size	50ug, 100ug, 200ug
Product Description	The Human INDO Protein(KMP2319) is produced in E.coli and the target gene encoding Met1-Gly403 is expressed with a 6His tag at the N-terminus.
Molecular Name	INDO
Product Introduction	INDO（IDO1）：色氨酸代谢限速酶，催化色氨酸沿犬尿氨酸途径的降解反应。
Molecular Weight	46.16 kDa
Expression System	E. coli
Species	Human
Purity	>95%
Purification	Affinity Purification
Uniprot ID	P14902
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	20mM Sodium Acetate, 150mM NaCl and 20% Glycerol, pH4.5
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	Indoleamine 2,3-dioxygenase(IDO) is a heme enzyme that initiates the oxidative degradation of the least abundant, essential amino acid, l-tryptophan, along the kynurenine pathway. This protein is normally expressed in the dendritic cells, macrophages, microglia, eosinophils, fibroblasts, endothelial cells, and most tumor cells. IDO activity is associated with immunosuppression and immune attenuation. Several studies showed that IDO can contribute to immune escape when expressed directly in tumor cells or when expressed in immunosuppressive antigen presenting cells such as tolerogenic dendritic cells or tumor associated macrophages. IDO also is a promising therapeutic target for the treatment of cancer, chronic viral infections, and other diseases characterized by pathological immune suppression.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

如何避免重组蛋白降解？:可在纯化过程中加入蛋白酶抑制剂、低温操作，优化储存条件（如分装冻存于-80°C），并避免反复冻融。某些情况下，可选用蛋白酶缺陷型宿主菌减少降解风险。
如何检测重组蛋白的表达和纯度？:常用SDS-PAGE、Western Blot检测表达，BCA/Bradford法测浓度，HPLC或质谱分析纯度，生物活性实验（如ELISA、酶活检测）验证功能。

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