Human CD47 Protein, Fc Tag, Avi Tag_天津卡梅德科技发展有限公司

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Human CD47 Protein, Fc Tag, Avi Tag

Human CD47 Protein, Fc Tag, Avi Tag

产品编号

KMP2305

别名

整联蛋白相关蛋白, Leukocyte Surface Antigen CD47, MER6

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Catalog Number	KMP2305
Alias	整联蛋白相关蛋白, Leukocyte Surface Antigen CD47, MER6
Size	50ug, 100ug, 200ug
Product Description	The Human CD47 Protein(KMP2305) is produced in HEK293 Cells and the target gene encoding Gln19-Pro139 is expressed with a Fc, Avi tag at the C-terminus.
Molecular Name	CD47
Product Introduction	CD47：免疫球蛋白超家族跨膜蛋白，广泛表达于多种细胞表面，通过结合SIRPα参与自我识别信号传递。
Molecular Weight	13.73 kDa
Expression System	HEK293 Cells
Species	Human
Purity	>95%
Purification	Affinity Purification
Uniprot ID	A0A0A1TSG4#structure
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	20mM PB, 150mM NaCl, pH7.4
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	CD47(Integrin-Associated Protein,IAP) is a 40 - 60 kDa variably glycosylated atypical member of the immunoglobulin superfamily. The ubiquitously expressed CD47 binds to SIRP family members on macrophages, neutrophils, and T cells. CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The protein is also a receptor for the C-terminal cell-binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This protein has broad tissue distribution, and is reduced in expression on Rh erythrocytes.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

蛋白溶解后浓度偏低，如何调整？

低速离心：12,000 rpm, 5分钟，去除不溶物。缓冲液置换：使用超滤离心管（如10 kDa截留分子量）浓缩。
如何提高重组蛋白的可溶性表达？

可优化表达条件（如降低诱导温度、调整IPTG浓度）、使用促溶标签（如SUMO、GST）、共表达分子伴侣，或尝试不同宿主系统（如哺乳动物细胞或昆虫细胞）以提高可溶性蛋白产量。

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