Mouse CD152 Protein, His Tag_天津卡梅德科技发展有限公司

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Mouse CD152 Protein, His Tag

Mouse CD152 Protein, His Tag

产品编号

KMP2299

别名

细胞毒T淋巴细胞相关抗原, Cytotoxic T-lymphocyte protein 4, CD152

规格

50ug
100ug
200ug

DATASHEET MSDS

立即询价加入询价列表大包装定制

产品介绍

Catalog Number	KMP2299
Alias	细胞毒T淋巴细胞相关抗原, Cytotoxic T-lymphocyte protein 4, CD152
Size	50ug, 100ug, 200ug
Product Description	The Mouse CD152 Protein(KMP2299) is produced in HEK293 Cells and the target gene encoding Ala37-Asp161 is expressed with a 6His tag at the C-terminus.
Molecular Name	CD152
Product Introduction	CD152（CTLA-4）：免疫调节受体，以跨膜形式表达于T细胞，参与免疫应答的负向调控。
Molecular Weight	14.56 kDa
Expression System	HEK293 Cells
Species	Mouse
Purity	>95%
SDS-PAGE
Purification	Affinity Purification
Uniprot ID	P09793
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	20mM PB, 150mM NaCl, pH7.4
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	Mouse Cytotoxic Tlymphocyte 4(CTLA-4,CD152), is a type I transmembrane T cell inhibitory molecule. mouse CTLA4 cDNA encodes 223 amino acids(aa) including a 35 aa signal sequence, a 126 aa extracellular domain(ECD) with one Ig-like V-type domain, a 21 aa transmembrane(TM) sequence, and a 41 aa cytoplasmic sequence. Within the ECD, Mouse CTLA-4 shares 68% aa sequence identity with human. CTLA4 is similar to the T cell costimulatory protein CD28 since both of the molecules bind to CD80 and CD86 on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found inregulatory T cells and may play an important role in their functions. T cell activation through the T cell receptor and CD28 leads to increased expression of CTLA4. Genetic variations of CTLA4 have been associated with susceptibility to systemic lupus erythematosus(SLE), Gravesdisease(GRD), Celiac disease type3(CELIAC3) and Hepatitis B virus infection(HBVinfection).
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

如何提高重组蛋白的可溶性表达？

可优化表达条件（如降低诱导温度、调整IPTG浓度）、使用促溶标签（如SUMO、GST）、共表达分子伴侣，或尝试不同宿主系统（如哺乳动物细胞或昆虫细胞）以提高可溶性蛋白产量。
重组蛋白纯化常用的方法有哪些？

常用的纯化方法包括亲和层析（如His-Tag、GST-Tag）、离子交换层析、疏水相互作用层析（HIC）和凝胶过滤层析。具体方法取决于蛋白特性和标签类型。

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