Human CD28 Protein, mFc Tag_天津卡梅德科技发展有限公司

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Human CD28 Protein, mFc Tag

Human CD28 Protein, mFc Tag

产品编号

KMP2290

别名

T细胞特异性表面糖蛋白CD28, CD28 molecule, Tp44

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Catalog Number	KMP2290
Alias	T细胞特异性表面糖蛋白CD28, CD28 molecule, Tp44
Size	50ug, 100ug, 200ug
Product Description	The Human CD28 Protein(KMP2290) is produced in HEK293 Cells and the target gene encoding Asn19-Pro152 is expressed with a mFc tag at the C-terminus.
Molecular Name	CD28
Product Introduction	CD28：免疫球蛋白超家族（IgSF）成员，以跨膜形式作为重要的共刺激受体参与T细胞活化。
Molecular Weight	15.15 kDa
Expression System	HEK293 Cells
Species	Human
Purity	>95%
Purification	Affinity Purification
Uniprot ID	B4E0L1
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	PBS, pH7.4
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	T-cell-specific surface glycoprotein CD28(CD28) is a single-pass typeI membrane protein which contains one Ig-likeV-type(immunoglobulin-like) domain. It belongs to the immunoglobulin(Ig) superfamily. CD28 is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation.CD28 co-stimulation is necessary for CD4 positive T-cell proliferation and survival, interleukin-2 production, and T-helper type-2 development. Human post-thymic regulatory T cells require CD28 co-stimulation to expand and maintain potent suppressive function in vivo. Apoptosis plays a key role in the age-related decline of CD28 expression and in immunosenescence. CD28 is the receptor for CD80(B7.1) and CD86(B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells(APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

如何避免重组蛋白降解？

可在纯化过程中加入蛋白酶抑制剂、低温操作，优化储存条件（如分装冻存于-80°C），并避免反复冻融。某些情况下，可选用蛋白酶缺陷型宿主菌减少降解风险。
如何检测重组蛋白的表达和纯度？

常用SDS-PAGE、Western Blot检测表达，BCA/Bradford法测浓度，HPLC或质谱分析纯度，生物活性实验（如ELISA、酶活检测）验证功能。

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