Human CD47 Protein, mFc Tag_天津卡梅德科技发展有限公司

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Human CD47 Protein, mFc Tag

Human CD47 Protein, mFc Tag

产品编号

KMP2289

别名

整联蛋白相关蛋白, Leukocyte Surface Antigen CD47, MER6

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Catalog Number	KMP2289
Alias	整联蛋白相关蛋白, Leukocyte Surface Antigen CD47, MER6
Size	50ug, 100ug, 200ug
Product Description	The Human CD47 Protein(KMP2289) is produced in HEK293 Cells and the target gene encoding Gln19-Pro139 is expressed with a mFc tag at the C-terminus.
Molecular Name	CD47
Product Introduction	CD47：免疫球蛋白超家族跨膜蛋白，广泛表达于多种细胞表面，通过结合SIRPα参与自我识别信号传递。
Molecular Weight	14.72 kDa
Expression System	HEK293 Cells
Species	Human
Purity	>95%
SDS-PAGE
Purification	Affinity Purification
Uniprot ID	Q08722#function
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	PBS, pH7.4
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	CD47(Integrin-Associated Protein,IAP) is a 40 - 60 kDa variably glycosylated atypical member of the immunoglobulin superfamily. The ubiquitously expressed CD47 binds to SIRP family members on macrophages, neutrophils, and T cells. CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The protein is also a receptor for the C-terminal cell-binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This protein has broad tissue distribution, and is reduced in expression on Rh erythrocytes.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

如何避免重组蛋白降解？

可在纯化过程中加入蛋白酶抑制剂、低温操作，优化储存条件（如分装冻存于-80°C），并避免反复冻融。某些情况下，可选用蛋白酶缺陷型宿主菌减少降解风险。
如何检测重组蛋白的表达和纯度？

常用SDS-PAGE、Western Blot检测表达，BCA/Bradford法测浓度，HPLC或质谱分析纯度，生物活性实验（如ELISA、酶活检测）验证功能。

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