Human CD96 Protein, mFc Tag_天津卡梅德科技发展有限公司

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Human CD96 Protein, mFc Tag

Human CD96 Protein, mFc Tag

产品编号

KMP2286

别名

T细胞表面蛋白触觉, T-cell surface protein tactile, CD96

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Catalog Number	KMP2286
Alias	T细胞表面蛋白触觉, T-cell surface protein tactile, CD96
Size	50ug, 100ug, 200ug
Product Description	The Human CD96 Protein(KMP2286) is produced in HEK293 Cells and the target gene encoding Val22-Met503 is expressed with a mFc tag at the C-terminus.
Molecular Name	CD96
Product Introduction	CD96：免疫球蛋白超家族跨膜蛋白，主要表达于NK细胞和T细胞，参与免疫细胞的功能调节。
Molecular Weight	53.02 kDa
Expression System	HEK293 Cells
Species	Human
Purity	>90%
SDS-PAGE
Purification	Affinity Purification
Uniprot ID	U3KPT0
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	PBS, pH7.4
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	The cluster of differentiation(CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. The CD155 ligand CD96 is a member of the Ig superfamily. It's a immunoglobulin-like protein tentatively allocated to the repertoire of human NK receptors. NK cells recognize poliovirus receptor(PVR), anectins and nectin-like protein family member serve to mediate cell-cell adhesion, cell migration, with the presence of an additional receptor, CD96. CD96 promotes NK cell adhesion to target cells expressing PVR, stimulates cytotoxicity of activated NK cells, and mediates acquisition of PVR from target cells.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

蛋白溶解后浓度偏低，如何调整？

低速离心：12,000 rpm, 5分钟，去除不溶物。缓冲液置换：使用超滤离心管（如10 kDa截留分子量）浓缩。
如何提高重组蛋白的可溶性表达？

可优化表达条件（如降低诱导温度、调整IPTG浓度）、使用促溶标签（如SUMO、GST）、共表达分子伴侣，或尝试不同宿主系统（如哺乳动物细胞或昆虫细胞）以提高可溶性蛋白产量。

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