Human CD40L Protein, His Tag_天津卡梅德科技发展有限公司

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Human CD40L Protein, His Tag

Human CD40L Protein, His Tag

产品编号

KMP2284

别名

肿瘤坏死因子相关激活蛋白, Tumor Necrosis Factor Ligand Superfamily Member 5, CD154

规格

50ug
100ug
200ug

DATASHEET MSDS

立即询价加入询价列表大包装定制

产品介绍

Catalog Number	KMP2284
Alias	肿瘤坏死因子相关激活蛋白, Tumor Necrosis Factor Ligand Superfamily Member 5, CD154
Size	50ug, 100ug, 200ug
Product Description	The Human CD40L Protein(KMP2284) is produced in HEK293 Cells and the target gene encoding Glu108-Leu261 is expressed with a 6His tag at the N-terminus.
Molecular Name	CD40L
Product Introduction	CD40L（CD154）：一种II型跨膜蛋白，作为CD40的天然配体，在活化T细胞表面表达，参与B细胞活化和抗体产生。
Molecular Weight	17.62 kDa
Expression System	HEK293 Cells
Species	Human
Purity	>95%
Purification	Affinity Purification
Uniprot ID	P25942
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	PBS, 1mM EDTA, pH7.4
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	CD40 Ligand(CD40LG) is a type II transmembrane glycoprotein that belongs to the TNF superfamily. Like other TNF superfamily members, CD40LG exists as a trimer in membrane bound and soluble form, both of which are bioactive. CD40LG is a ligand for CD40; its ligation also initiates signal transduction in CD40LG expressing cells. CD40LG is a differentiation antigen that is expressed on the surface of T-cells. It stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40LG has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

如何提高重组蛋白的可溶性表达？

可优化表达条件（如降低诱导温度、调整IPTG浓度）、使用促溶标签（如SUMO、GST）、共表达分子伴侣，或尝试不同宿主系统（如哺乳动物细胞或昆虫细胞）以提高可溶性蛋白产量。
重组蛋白纯化常用的方法有哪些？

常用的纯化方法包括亲和层析（如His-Tag、GST-Tag）、离子交换层析、疏水相互作用层析（HIC）和凝胶过滤层析。具体方法取决于蛋白特性和标签类型。

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