Human CD155 Protein, mFc Tag_天津卡梅德科技发展有限公司

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Human CD155 Protein, mFc Tag

Human CD155 Protein, mFc Tag

产品编号

KMP2282

别名

重组人脊髓灰质炎病毒受体, Poliovirus Receptor, CD155

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Catalog Number	KMP2282
Alias	重组人脊髓灰质炎病毒受体, Poliovirus Receptor, CD155
Size	50ug, 100ug, 200ug
Product Description	The Human CD155 Protein(KMP2282) is produced in HEK293 Cells and the target gene encoding Trp21-Asn343 is expressed with a mFc tag at the C-terminus.
Molecular Name	CD155
Product Introduction	CD155（PVR）：免疫球蛋白超家族（IgSF）成员，通过跨膜形式参与细胞间相互作用和免疫调节。
Molecular Weight	35.08 kDa
Expression System	HEK293 Cells
Species	Human
Purity	>95%
Purification	Affinity Purification
Uniprot ID	P15151
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	PBS, pH7.4
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	Poliovirus Receptor(PVR) is a 70 kDa type I transmembrane single-span glycoprotein that belongs to the nectin-like(Necl) family and was originally identified based on its ability to mediate the cell attachment and entry of poliovirus(PV), an etiologic agent of the central nervous system disease poliomyelitis. PVR contains three Ig-like extracellular domains, a transmembrane segment, and a cytoplasmic tail. The normal cellular function of PVR maybe the involvement of intercellular adhension between epithelial cells. Alternate splicing of the PVR mRNA yields four different isoforms(α, β, γ, and δ) with identical extracellular domains.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

如何避免重组蛋白降解？

可在纯化过程中加入蛋白酶抑制剂、低温操作，优化储存条件（如分装冻存于-80°C），并避免反复冻融。某些情况下，可选用蛋白酶缺陷型宿主菌减少降解风险。
如何检测重组蛋白的表达和纯度？

常用SDS-PAGE、Western Blot检测表达，BCA/Bradford法测浓度，HPLC或质谱分析纯度，生物活性实验（如ELISA、酶活检测）验证功能。

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