Human CD258 Protein, mFc Tag_天津卡梅德科技发展有限公司

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Human CD258 Protein, mFc Tag

Human CD258 Protein, mFc Tag

产品编号

KMP2281

别名

肿瘤坏死因子配体超家族成员 14, Tumor necrosis factor ligand superfamily member 14, CD258

规格

50ug
100ug
200ug

DATASHEET MSDS

立即询价加入询价列表大包装定制

产品介绍

Catalog Number	KMP2281
Alias	肿瘤坏死因子配体超家族成员 14, Tumor necrosis factor ligand superfamily member 14, CD258
Size	50ug, 100ug, 200ug
Product Description	The Human CD258 Protein(KMP2281) is produced in HEK293 Cells and the target gene encoding Ile84-Val240 is expressed with a mFc tag at the N-terminus.
Molecular Name	CD258
Product Introduction	CD258（LIGHT）：TNF超家族成员，以跨膜形式参与淋巴组织发育和免疫调节。
Molecular Weight	17.17 kDa
Expression System	HEK293 Cells
Species	Human
Purity	>95%
Purification	Affinity Purification
Uniprot ID	O43557
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	PBS, pH7.4
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	Human TNFSF14 Protein, also known as LIGHT, belongs to a member of the tumor necrosis factor(TNF) ligand family. It can bind to NFRSF3/LTBR. It is a ligand for TNFRSF14, which is a member of the tumor necrosis factor receptor superfamily, and it is also known as a herpesvirus entry mediator ligand(HVEML). TNFSF14 encodes a protein with a 37 aa cytoplasmic domain, 21aa transmembrane domain and 182 aa extracellular region. The gene is predominantly expressed in the spleen and also found in the brain. Weakly expressed in peripheral lymphoid tissues and in heart, placenta, liver, lung, appendix, and kidney, and no expression seen in fetal tissues, endocrine glands, or nonhematopoietic tumor lines. TNFSF14 protein was found to probably function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. Studies have shown that this protein can prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

蛋白溶解后浓度偏低，如何调整？

低速离心：12,000 rpm, 5分钟，去除不溶物。缓冲液置换：使用超滤离心管（如10 kDa截留分子量）浓缩。
如何提高重组蛋白的可溶性表达？

可优化表达条件（如降低诱导温度、调整IPTG浓度）、使用促溶标签（如SUMO、GST）、共表达分子伴侣，或尝试不同宿主系统（如哺乳动物细胞或昆虫细胞）以提高可溶性蛋白产量。

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