Human CD273 Protein, His Tag, Avi Tag_天津卡梅德科技发展有限公司

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Human CD273 Protein, His Tag, Avi Tag

Human CD273 Protein, His Tag, Avi Tag

产品编号

KMP2276

别名

程序性细胞死亡 1 配体 2, Programmed Cell Death 1 Ligand 2, CD273

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Catalog Number	KMP2276
Alias	程序性细胞死亡 1 配体 2, Programmed Cell Death 1 Ligand 2, CD273
Size	50ug, 100ug, 200ug
Product Description	The Human CD273 Protein(KMP2276) is produced in HEK293 Cells and the target gene encoding Leu20-Pro219 is expressed with a 6His, Avi tag at the C-terminus.
Molecular Name	CD273
Product Introduction	CD273（PD-L2）：免疫球蛋白超家族（IgSF）成员，以跨膜形式参与免疫细胞间的信号传导。
Molecular Weight	23.38 kDa
Expression System	HEK293 Cells
Species	Human
Purity	>95%
SDS-PAGE
Purification	Affinity Purification
Uniprot ID	Q9BQ51
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	20mM PB, 150mM NaCl, pH7.4
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	Programmed Cell Death 1 Ligand 2(PDCD1LG2) is a member of the BTN/MOG family. PDCD1LG2 contains one Ig-like C2-type domain and one Ig-like V-type domain. PDCD1LG2 is highly expressed in the heart, placenta, pancreas, lung and liver; it is weakly expressed in the spleen, lymph nodes, and thymus. PDCD1LG2 is involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. PDCD1LG2 interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

如何避免重组蛋白降解？

可在纯化过程中加入蛋白酶抑制剂、低温操作，优化储存条件（如分装冻存于-80°C），并避免反复冻融。某些情况下，可选用蛋白酶缺陷型宿主菌减少降解风险。
如何检测重组蛋白的表达和纯度？

常用SDS-PAGE、Western Blot检测表达，BCA/Bradford法测浓度，HPLC或质谱分析纯度，生物活性实验（如ELISA、酶活检测）验证功能。

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