Human Biotinylated ROR1 Protein, His Tag, Avi Tag_天津卡梅德科技发展有限公司

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Human Biotinylated ROR1 Protein, His Tag, Avi Tag

Human Biotinylated ROR1 Protein, His Tag, Avi Tag

产品编号

KMP2275

别名

受体酪氨酸激酶样孤儿受体, Inactive tyrosine-protein kinase transmembrane receptor ROR1, ROR1

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Catalog Number	KMP2275
Alias	受体酪氨酸激酶样孤儿受体, Inactive tyrosine-protein kinase transmembrane receptor ROR1, ROR1
Size	50ug, 100ug, 200ug
Product Description	The Human Biotinylated ROR1 Protein(KMP2275) is produced in HEK293 Cells and the target gene encoding Gln30-Glu403 is expressed with a 6His, Avi tag at the C-terminus.
Molecular Name	ROR1
Product Introduction	ROR1：受体酪氨酸激酶家族跨膜蛋白，在胚胎发育过程中发挥重要作用。
Molecular Weight	42.82 kDa
Expression System	HEK293 Cells
Species	Human
Purity	>95%
SDS-PAGE
Purification	Affinity Purification
Uniprot ID	Q01973
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	PBS, pH7.4
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	Receptor tyrosine kinase-like orphan receptor 1(ROR1), also known as neurotrophic tyrosine kinase, it is a member of the ROR family within receptor tyrosine kinases(RTK) superfamily. Human ROR1 is a type I transmembrane protein with 937 amino acids(aa) in length. It contains a 29 aa signal sequence, a 377 aa extracellular domain(ECD), a 21 aa transmembrane segment, and a 510 aa cytoplasmic region. ROR1 expressed strongly in human heart, lung and kidney, but weakly in the CNS. At developmental stage, it expressed at high levels during early embryonic development. ROR1 has been shown to have very low kinase activity in vitro and is unlikely to function as a tyrosine kinase in vivo. It may act as a receptor for wnt ligand WNT5A which may result in the inhibition of WNT3A-mediated signaling.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

如何储存293F细胞中表达的蛋白？

蛋白可以在-80°C下储存以便长期保存。应分装并避免冻融循环，以保持蛋白的稳定性。
如何提高293F细胞中的蛋白表达效率？

可以通过优化质粒设计、使用高效转染试剂、优化细胞培养条件以及使用蛋白折叠伴侣或密码子优化来提高蛋白表达效率。

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