Human Biotinylated Mesothelin Protein, His Tag, Avi Tag_天津卡梅德科技发展有限公司

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Human Biotinylated Mesothelin Protein, His Tag, Avi Tag

Human Biotinylated Mesothelin Protein, His Tag, Avi Tag

产品编号

KMP2274

别名

间皮素, Megakaryocyte potentiating facto, Mesothelin

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Catalog Number	KMP2274
Alias	间皮素, Megakaryocyte potentiating facto, Mesothelin
Size	50ug, 100ug, 200ug
Product Description	The Human Biotinylated Mesothelin Protein(KMP2274) is produced in HEK293 Cells and the target gene encoding Glu296-Ser598 is expressed with a 6His, Avi tag at the C-terminus.
Molecular Name	Mesothelin
Product Introduction	Mesothelin：GPI锚定糖蛋白，在间皮细胞表面表达，参与细胞间黏附作用。
Molecular Weight	33.99 kDa
Expression System	HEK293 Cells
Species	Human
Purity	>95%
SDS-PAGE
Purification	Affinity Purification
Uniprot ID	H3BR90
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	PBS, pH7.4
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	Mesothelin is a cell surface glycoprotein whose expression is limited to mesothelial cells of the serosa(pleura, pericardium, and peritoneum) and epithelial cells of the trachea, tonsils, fallopian tube, and kidneys. Mesothelin plays an important role in cell survival, proliferation, migration, invasion, tumor progression, and resistance to chemotherapy. The overexpression of mesothelin can activate NF-κB and signal transducer and activator of transcription 3(Stat3), inhibit apoptotic signaling and TNF-α-induced apoptosis, and accelerate the G1–S transition. Mesothelin is also found overexpressed in various cancers, including malignant mesothelioma, pancreatic or ovarian carcinoma, sarcomas and in some gastrointestinal or pulmonary carcinomas. As a result of its limited expression in normal tissues, mesothelin has been reported as an ideal tumor-associated marker for the development of targeted therapy.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

蛋白溶解后浓度偏低，如何调整？

低速离心：12,000 rpm, 5分钟，去除不溶物。缓冲液置换：使用超滤离心管（如10 kDa截留分子量）浓缩。
如何提高重组蛋白的可溶性表达？

可优化表达条件（如降低诱导温度、调整IPTG浓度）、使用促溶标签（如SUMO、GST）、共表达分子伴侣，或尝试不同宿主系统（如哺乳动物细胞或昆虫细胞）以提高可溶性蛋白产量。

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