Human HER1 Protein, His Tag, Avi Tag_天津卡梅德科技发展有限公司

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Human HER1 Protein, His Tag, Avi Tag

Human HER1 Protein, His Tag, Avi Tag

产品编号

KMP2271

别名

表皮生长因子受体, Epidermal growth factor receptor, EGFR

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Catalog Number	KMP2271
Alias	表皮生长因子受体, Epidermal growth factor receptor, EGFR
Size	50ug, 100ug, 200ug
Product Description	The Human HER1 Protein(KMP2271) is produced in HEK293 Cells and the target gene encoding Leu25-Ser378 is expressed with a 6His, Avi tag at the C-terminus.
Molecular Name	HER1
Product Introduction	HER1（EGFR）：受体酪氨酸激酶家族跨膜蛋白，通过配体结合介导细胞增殖和分化信号。
Molecular Weight	39.6 kDa
Expression System	HEK293 Cells
Species	Human
Purity	>95%
Purification	Affinity Purification
Uniprot ID	P00533
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	PBS, pH7.4
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	The EGFR subfamily of receptor tyrosine kinases is composed of EGFR, ErbB2, ErbB3 and ErbB4. The EGFR shares 43% - 44% aa sequence identity with the ECD of human EGFR subfamily. All these family members are type I transmembrane glycoproteins with an extracellular ligand binding domain. The extracellular ligand binding domain is containing two cysteine-rich domains separated by a spacer region and a cytoplasmic domain containing a membrane-proximal tyrosine kinase domain. Ligand binding could induce EGFR homodimerization and heterodimerization with ErbB2, resulting in cell signaling, heterodimerization tyrosine phosphorylation and kinase activation. It can bind EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, HB-EGF, epigen, and so on. Its signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis. EGFR can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGFR is overexpressed in different tumors. Several anti-cancer drugs use EGFR as target.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

如何提高重组蛋白的可溶性表达？:可优化表达条件（如降低诱导温度、调整IPTG浓度）、使用促溶标签（如SUMO、GST）、共表达分子伴侣，或尝试不同宿主系统（如哺乳动物细胞或昆虫细胞）以提高可溶性蛋白产量。
重组蛋白纯化常用的方法有哪些？:常用的纯化方法包括亲和层析（如His-Tag、GST-Tag）、离子交换层析、疏水相互作用层析（HIC）和凝胶过滤层析。具体方法取决于蛋白特性和标签类型。

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