Mouse ILT3 Protein, Fc Tag_天津卡梅德科技发展有限公司

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Mouse ILT3 Protein, Fc Tag

Mouse ILT3 Protein, Fc Tag

产品编号

KMP2269

别名

白细胞免疫球蛋白样受体 B 亚家族成员 4, Leukocyte immunoglobulin-like receptor subfamily B member 4, ILT3

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Catalog Number	KMP2269
Alias	白细胞免疫球蛋白样受体 B 亚家族成员 4, Leukocyte immunoglobulin-like receptor subfamily B member 4, ILT3
Size	50ug, 100ug, 200ug
Product Description	The Mouse ILT3 Protein(KMP2269) is produced in HEK293 Cells and the target gene encoding Gly24-Lys238 is expressed with a Fc tag at the C-terminus.
Molecular Name	ILT3
Product Introduction	ILT3（LILRB4）：免疫球蛋白样转录抑制受体，以跨膜形式参与免疫抑制信号的传递。
Molecular Weight	23.65 kDa
Expression System	HEK293 Cells
Species	Mouse
Purity	>95%
Purification	Affinity Purification
Uniprot ID	Q64281
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	50mM Tris-HCl, 100mM Glycine, pH7.5
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	Mouse Leukocyte Immunoglobulin-like Receptor Subfamily B Member 4(LILRB4/CD85k/ILT3) is an approximately transmembrane glycoprotein that negatively regulates immune cell activation. Mouse LILRB4 consists of a 215 amino acid(aa) extracellular domain with two Ig-like domains, a 22 aa transmembrane segment, and a 75 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs(ITIM). Within the ECD, mouse LILRB4 shares 45% and 77% aa sequence identity with human and rat LILRB4, respectively. Alternative splicing of mouse LILRB4 generates a potentially soluble isoform that lacks the transmembrane segment. LILRB4 is expressed on dendritic cells(DC), monocytes, macrophages, and vascular endothelial cells(EC). Ligation of LILRB4 triggers ITIM-mediated inhibition of cellactivating signaling, leading to enhanced immune tolerance and reduced allogeneic graft rejection. Soluble LILRB4 induces the differentiation of CD8+ T suppressor cells(Ts) that can inhibit the effector functions of CD4+ Th cells and CD8+ CTL. In turn, CD8+ Ts cells induce LILRB4 up-regulation and a tolerogenic phenotype in monocytes, DC, and EC.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

如何避免重组蛋白降解？:可在纯化过程中加入蛋白酶抑制剂、低温操作，优化储存条件（如分装冻存于-80°C），并避免反复冻融。某些情况下，可选用蛋白酶缺陷型宿主菌减少降解风险。
如何检测重组蛋白的表达和纯度？:常用SDS-PAGE、Western Blot检测表达，BCA/Bradford法测浓度，HPLC或质谱分析纯度，生物活性实验（如ELISA、酶活检测）验证功能。

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