Human CD28 Protein, His Tag_天津卡梅德科技发展有限公司

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Human CD28 Protein, His Tag

Human CD28 Protein, His Tag

产品编号

KMP2267

别名

T细胞特异性表面糖蛋白CD28, CD28 molecule, Tp44

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Catalog Number	KMP2267
Alias	T细胞特异性表面糖蛋白CD28, CD28 molecule, Tp44
Size	50ug, 100ug, 200ug
Product Description	The Human CD28 Protein(KMP2267) is produced in HEK293 Cells and the target gene encoding Asn19-Pro152 is expressed with a 6His tag at the N-terminus.
Molecular Name	CD28
Product Introduction	CD28：免疫球蛋白超家族（IgSF）成员，以跨膜形式作为重要的共刺激受体参与T细胞活化。
Molecular Weight	15.97 kDa
Expression System	HEK293 Cells
Species	Human
Purity	>95%
Purification	Affinity Purification
Uniprot ID	Q96BF3
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	20mM Tris, 150mM NaCl, pH8.0
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	T-cell-specific surface glycoprotein CD28(CD28) is a single-pass typeI membrane protein which contains one Ig-likeV-type(immunoglobulin-like) domain. It belongs to the immunoglobulin(Ig) superfamily. CD28 is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation.CD28 co-stimulation is necessary for CD4 positive T-cell proliferation and survival, interleukin-2 production, and T-helper type-2 development. Human post-thymic regulatory T cells require CD28 co-stimulation to expand and maintain potent suppressive function in vivo. Apoptosis plays a key role in the age-related decline of CD28 expression and in immunosenescence. CD28 is the receptor for CD80(B7.1) and CD86(B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells(APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

如何提高重组蛋白的可溶性表达？

可优化表达条件（如降低诱导温度、调整IPTG浓度）、使用促溶标签（如SUMO、GST）、共表达分子伴侣，或尝试不同宿主系统（如哺乳动物细胞或昆虫细胞）以提高可溶性蛋白产量。
重组蛋白纯化常用的方法有哪些？

常用的纯化方法包括亲和层析（如His-Tag、GST-Tag）、离子交换层析、疏水相互作用层析（HIC）和凝胶过滤层析。具体方法取决于蛋白特性和标签类型。

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