Human CD33L3 Protein, mFc Tag_天津卡梅德科技发展有限公司

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Human CD33L3 Protein, mFc Tag

Human CD33L3 Protein, mFc Tag

产品编号

KMP2265

别名

唾液酸结合免疫球蛋白样凝集素-15, Sialic acid-binding Ig-like lectin 15, Siglec-15

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Catalog Number	KMP2265
Alias	唾液酸结合免疫球蛋白样凝集素-15, Sialic acid-binding Ig-like lectin 15, Siglec-15
Size	50ug, 100ug, 200ug
Product Description	The Human CD33L3 Protein(KMP2265) is produced in HEK293 Cells and the target gene encoding Phe20-Thr263 is expressed with a mFc tag at the C-terminus.
Molecular Name	CD33L3
Product Introduction	CD33L3（Siglec-3L）：免疫球蛋白超家族（IgSF）成员，通过跨膜形式参与髓系细胞功能调节。
Molecular Weight	26.56 kDa
Expression System	HEK293 Cells
Species	Human
Purity	>95%
SDS-PAGE
Purification	Affinity Purification
Uniprot ID	A7E1W7
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	PBS, 150mM Nacl, 0.3% Chaps, 5% Trehalose, pH7.4
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	Human Siglec-15 is a transmembrane glycoprotein in the Siglec family. Siglecs are type I transmembrane proteins where the NH3+-terminus is in the extracellular space and the COO--terminus is cytosolic. Each Siglec contains an N-terminal V-type immunoglobulin domain(Ig domain) which acts as the binding receptor for sialic acid. These lectins are placed into the group of I-type lectins because the lectin domain is an immunoglobulin fold. All Siglecs are extended from the cell surface by C2-type Ig domains which have no binding activity. Siglecs differ in the number of these C2-type domains. Human Siglec-15 consists of a 244 amino acid(aa) extracellular domain(ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

蛋白溶解后浓度偏低，如何调整？

低速离心：12,000 rpm, 5分钟，去除不溶物。缓冲液置换：使用超滤离心管（如10 kDa截留分子量）浓缩。
如何提高重组蛋白的可溶性表达？

可优化表达条件（如降低诱导温度、调整IPTG浓度）、使用促溶标签（如SUMO、GST）、共表达分子伴侣，或尝试不同宿主系统（如哺乳动物细胞或昆虫细胞）以提高可溶性蛋白产量。

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