Human Biotinylated FGL1 Protein, Fc Tag, Avi Tag_天津卡梅德科技发展有限公司

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Human Biotinylated FGL1 Protein, Fc Tag, Avi Tag

Human Biotinylated FGL1 Protein, Fc Tag, Avi Tag

产品编号

KMP2257

别名

纤维蛋白原样 1, Fibrinogen-like protein 1, FGL1

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Catalog Number	KMP2257
Alias	纤维蛋白原样 1, Fibrinogen-like protein 1, FGL1
Size	50ug, 100ug, 200ug
Product Description	The Human Biotinylated FGL1 Protein(KMP2257) is produced in HEK293 Cells and the target gene encoding Leu23-Ile312 is expressed with a Fc, Avi tag at the C-terminus.
Molecular Name	FGL1
Product Introduction	FGL1：分泌型纤维蛋白原样蛋白，主要由肝脏产生，参与组织修复和免疫调节过程。
Molecular Weight	34.02 kDa
Expression System	HEK293 Cells
Species	Human
Purity	>90%
SDS-PAGE
Purification	Affinity Purification
Uniprot ID	D3DSP9
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	PBS, 5% Trehalose, pH7.4
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	Fibrinogen-like protein 1(FGL1) is also known as HP-041, Hepassocin, HFREP-1, LFIRE-1, is a liver-specific secreted protein belonging to the fibronogen superfamily, whose members share a fibrinogen domain at their C-termini. It is secreted by the liver and functions as a mitogen for hepatocytes. Hepassocin may play a role in the development of hepatocellular carcinomas. Hepassocin is a disulfide-linked homodimeric protein with a C-terminal fibrinogen domain. It is reported that it is a major immune inhibitory ligand of LAG-3.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

如何避免重组蛋白降解？

可在纯化过程中加入蛋白酶抑制剂、低温操作，优化储存条件（如分装冻存于-80°C），并避免反复冻融。某些情况下，可选用蛋白酶缺陷型宿主菌减少降解风险。
如何检测重组蛋白的表达和纯度？

常用SDS-PAGE、Western Blot检测表达，BCA/Bradford法测浓度，HPLC或质谱分析纯度，生物活性实验（如ELISA、酶活检测）验证功能。

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