Cynomolgus Biotinylated FGL1 Protein, Avi Tag, His Tag_天津卡梅德科技发展有限公司

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Cynomolgus Biotinylated FGL1 Protein, Avi Tag, His Tag

Cynomolgus Biotinylated FGL1 Protein, Avi Tag, His Tag

产品编号

KMP2256

别名

纤维蛋白原样 1, Fibrinogen-like protein 1, FGL1

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Catalog Number	KMP2256
Alias	纤维蛋白原样 1, Fibrinogen-like protein 1, FGL1
Size	50ug, 100ug, 200ug
Product Description	The Cynomolgus Biotinylated FGL1 Protein(KMP2256) is produced in HEK293 Cells and the target gene encoding Leu23-Ile312 is expressed with a AVI, 6His tag at the C-terminus.
Molecular Name	FGL1
Product Introduction	FGL1：分泌型纤维蛋白原样蛋白，主要由肝脏产生，参与组织修复和免疫调节过程。
Molecular Weight	32.56 kDa
Expression System	HEK293 Cells
Species	Cynomolgus
Purity	>95%
SDS-PAGE
Purification	Affinity Purification
Uniprot ID	A0A2K5X990
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	20mM Tris, 300mM NaCl, 10% Glycerol, 2mM EDTA, pH8.0
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	Hepassocin, also known as hepatocyte-derived fibrinogen-related protein 1(HFREP-1), is a liver-specific secreted protein belonging to the fibronogen superfamily, whose members share a fibrinogen domain at their C-termini. It is secreted by the liver and functions as a mitogen for hepatocytes. Hepassocin may play a role in the development of hepatocellular carcinomas. Hepassocin is a disulfide-linked homodimeric protein with a C-terminal fibrinogen domain. It is reported that it is a major immune inhibitory ligand of LAG-3.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

如何提高重组蛋白的可溶性表达？

可优化表达条件（如降低诱导温度、调整IPTG浓度）、使用促溶标签（如SUMO、GST）、共表达分子伴侣，或尝试不同宿主系统（如哺乳动物细胞或昆虫细胞）以提高可溶性蛋白产量。
重组蛋白纯化常用的方法有哪些？

常用的纯化方法包括亲和层析（如His-Tag、GST-Tag）、离子交换层析、疏水相互作用层析（HIC）和凝胶过滤层析。具体方法取决于蛋白特性和标签类型。

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