Human Tie-2 Protein, Fc Tag_天津卡梅德科技发展有限公司

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Human Tie-2 Protein, Fc Tag

Human Tie-2 Protein, Fc Tag

产品编号

KMP2248

别名

血管生成素-1受体, angiopoietin-1 receptor, CD202b

规格

50ug
100ug
200ug

DATASHEET MSDS

立即询价加入询价列表大包装定制

产品介绍

Catalog Number	KMP2248
Alias	血管生成素-1受体, angiopoietin-1 receptor, CD202b
Size	50ug, 100ug, 200ug
Product Description	The Human Tie-2 Protein(KMP2248) is produced in HEK293 Cells and the target gene encoding Ala23-Leu748 is expressed with a Fc tag at the C-terminus.
Molecular Name	Tie-2
Product Introduction	Tie-2：血管内皮生长因子受体家族成员，特异性表达于内皮细胞，参与血管稳态维持。
Molecular Weight	81.03 kDa
Expression System	HEK293 Cells
Species	Human
Purity	>95%
Purification	Affinity Purification
Uniprot ID	Q02763
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	PBS, pH7.4
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	Tie-1/Tie(tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase(RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor(EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Human Tie-2 cDNA encodes a 1124 amino acid(aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Two ligands, angiopoietin-1(Ang-1) and angiopoietin-2(Ang-2), which bind Tie-2 with high-affinity have been identified. Ang-2 has been reported to act as an antagonist for Ang-1. Mice engineered to overexpress Ang-2 or to lack Ang-1 or Tie-2 display similar angiogenesis defects.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

蛋白溶解后浓度偏低，如何调整？:低速离心：12,000 rpm, 5分钟，去除不溶物。缓冲液置换：使用超滤离心管（如10 kDa截留分子量）浓缩。
如何提高重组蛋白的可溶性表达？:可优化表达条件（如降低诱导温度、调整IPTG浓度）、使用促溶标签（如SUMO、GST）、共表达分子伴侣，或尝试不同宿主系统（如哺乳动物细胞或昆虫细胞）以提高可溶性蛋白产量。

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