Human CD172a Protein, His Tag_天津卡梅德科技发展有限公司

>>

>>

>>

>

Human CD172a Protein, His Tag

Human CD172a Protein, His Tag

产品编号

KMP2246

别名

信号调节蛋白, CD172a, SHPS-1

规格

50ug
100ug
200ug

DATASHEET MSDS

立即询价加入询价列表大包装定制

产品介绍

Catalog Number	KMP2246
Alias	信号调节蛋白, CD172a, SHPS-1
Size	50ug, 100ug, 200ug
Product Description	The Human CD172a Protein(KMP2246) is produced in HEK293 Cells and the target gene encoding Glu31-Arg370 is expressed with a 6His tag at the C-terminus.
Molecular Name	CD172a
Product Introduction	CD172a（SIRPα）：免疫球蛋白超家族（IgSF）成员，主要表达于髓系细胞，参与细胞间识别和信号转导。
Molecular Weight	38.14 kDa
Expression System	HEK293 Cells
Species	Human
Purity	>95%
SDS-PAGE
Purification	Affinity Purification
Uniprot ID	P78324
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	20mM PB, 150mM NaCl, pH7.2
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	Signal Regulatory Protein α(SIRPα) is a monomeric approximately 90 kD type I transmembrane glycoprotein. The 504 amino acid human SIRPα contains two Ig-like C1-type domains and one Ig-like V-type domain. SIRPα can express in various tissues, mainly on brain and myeloid cells, including macrophages, neutrophils, dendritic and Langerhans cells. It also can detect in neurons, smooth muscle and endothelial cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPα acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPα shows adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. SIRPα engagement generally produces a negative regulatory signal; it may mediate negative regulation of phagocytosis, mast cell activation and dendritic cell activation
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

如何提高重组蛋白的可溶性表达？

可优化表达条件（如降低诱导温度、调整IPTG浓度）、使用促溶标签（如SUMO、GST）、共表达分子伴侣，或尝试不同宿主系统（如哺乳动物细胞或昆虫细胞）以提高可溶性蛋白产量。
重组蛋白纯化常用的方法有哪些？

常用的纯化方法包括亲和层析（如His-Tag、GST-Tag）、离子交换层析、疏水相互作用层析（HIC）和凝胶过滤层析。具体方法取决于蛋白特性和标签类型。

推荐产品

400-621-6806
18630936064（微信同号）
周一至周五 8:30-18:00
0