Human CD73 Protein, Fc Tag_天津卡梅德科技发展有限公司

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Human CD73 Protein, Fc Tag

Human CD73 Protein, Fc Tag

产品编号

KMP2245

别名

胞外-5′-核苷酸酶, 5'-Nucleotidase, NT5E

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Catalog Number	KMP2245
Alias	胞外-5′-核苷酸酶, 5'-Nucleotidase, NT5E
Size	50ug, 100ug, 200ug
Product Description	The Human CD73 Protein(KMP2245) is produced in HEK293 Cells and the target gene encoding Trp27-Lys547 is expressed with a Fc tag at the C-terminus.
Molecular Name	CD73
Product Introduction	CD73：外-5'-核苷酸酶，以GPI锚定形式表达于细胞膜，催化AMP水解参与嘌呤代谢调控。
Molecular Weight	57.79 kDa
Expression System	HEK293 Cells
Species	Human
Purity	>95%
SDS-PAGE
Purification	Affinity Purification
Uniprot ID	P21589
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	20mM TrisHCl, 120mM NaCl, 4mM CaCl2, 20% Glycerol, pH7.5
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	CD73 is a glycosyl phosphatidylinositol(GPI) anchored membrane protein that belongs to the 5'-nucleotidase family. CD73 is an ecto 5'Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'Nucleotidase activity. Defects in NT5E are the cause of calcification of joints and arteries(CAJA). The recombinant CD73 lacking GPI anchor is secreted as a monomer.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

如何提高重组蛋白的可溶性表达？

可优化表达条件（如降低诱导温度、调整IPTG浓度）、使用促溶标签（如SUMO、GST）、共表达分子伴侣，或尝试不同宿主系统（如哺乳动物细胞或昆虫细胞）以提高可溶性蛋白产量。
重组蛋白纯化常用的方法有哪些？

常用的纯化方法包括亲和层析（如His-Tag、GST-Tag）、离子交换层析、疏水相互作用层析（HIC）和凝胶过滤层析。具体方法取决于蛋白特性和标签类型。

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