Human Siglec-10 Protein, mFc Tag_天津卡梅德科技发展有限公司

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Human Siglec-10 Protein, mFc Tag

Human Siglec-10 Protein, mFc Tag

产品编号

KMP2239

别名

唾液酸结合免疫球蛋白样凝集素-10, Siglec10, MGC126774

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Catalog Number	KMP2239
Alias	唾液酸结合免疫球蛋白样凝集素-10, Siglec10, MGC126774
Size	50ug, 100ug, 200ug
Product Description	The Human Siglec-10 Protein(KMP2239) is produced in HEK293 Cells and the target gene encoding Met17-Thr546 is expressed with a mFc tag at the C-terminus.
Molecular Name	Siglec-10
Product Introduction	Siglec-10：唾液酸结合性免疫球蛋白样凝集素，以跨膜形式参与免疫细胞的负向调控。
Molecular Weight	58.23 kDa
Expression System	HEK293 Cells
Species	Human
Purity	>90%
SDS-PAGE
Purification	Affinity Purification
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	PBS, pH7.4
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	Siglecs(sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system. Siglec-G is the apparent ortholog of human Siglec-10. We describe here a novel member of the siglec protein family that shares a similar structure including five Ig-like domains, a transmembrane domain, and a cytoplasmic tail containing two ITIM-signaling motifs. Siglec-10 was identified through database mining of an asthmatic eosinophil EST library. Siglec-10 binds sialated proteins and lipids in alpha 2,3 or alpha 2,6 linkage and shows a preference for GT1b gangliosides. This binding can be modulated by cis interactions of Siglec-10 with sialated molecules expressed on the same cell. When tyrosine phosphorylated, the cytoplasmic ITIMs interact with phosphatases SHP-1 and SHP-2 to propagate inhibitory signals. The Siglec-10-VAP-1 interaction seems to mediate lymphocyte adhesion to endothelium and has the potential to modify the inflammatory microenvironment via the enzymatic end products.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

如何避免重组蛋白降解？

可在纯化过程中加入蛋白酶抑制剂、低温操作，优化储存条件（如分装冻存于-80°C），并避免反复冻融。某些情况下，可选用蛋白酶缺陷型宿主菌减少降解风险。
如何检测重组蛋白的表达和纯度？

常用SDS-PAGE、Western Blot检测表达，BCA/Bradford法测浓度，HPLC或质谱分析纯度，生物活性实验（如ELISA、酶活检测）验证功能。

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