| Catalog Number | KMP1847 |
| Alias | 转化生长因子β1,TGF beta-1,TGFB1,TGF beta,TGFB,TGF beta 1,Transforming growth factor beta-1 proprotein,Human TGF beta 1 Protein, His Tag |
| Size | 20ug, 50ug, 100ug, 200ug, 1mg |
| Molecular Name | LAP, TGF beta 1 |
| Product Introduction | 转化生长因子β1是一种多功能分泌蛋白，通过Smad等信号通路精确调控细胞，在早期抑制上皮细胞增殖并促进后期纤维化与肿瘤发展。 |
| Molecular Weight | The protein has a predicted MW of 31.4 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Tris-Bis PAGE result. |
| Expression System | HEK293 Cells |
| Species | Human |
| Concentration | 联系销售经理获得最新批次的浓度 |
| Purity | > 95% as determined by Tris-Bis PAGE, > 95% as determined by HPLC |
| Uniprot ID | P01137 |
| Storage Condition | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Shipping Condition | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
| Background | Latent TGF beta 1 cDNA encodes a 390 amino acid precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin‑like convertase processes the proprotein to generate an N‑terminal 249 aa latency‑associated peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta 1. Disulfide‑linked homodimers of LAP and TGF‑ beta 1 remain non‑covalently associated after secretion, forming the small latent TGF‑ beta 1 complex. |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Application | SDS-PAGE, WB, ELISA |
| Tag | His tag |
| Biological Activity | Immobilized Human LAP (TGF beta 1), His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB6, His Tag with the EC50 of 1.0ug/ml determined by ELISA. |
| Predicted N Terminus | His |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Sequence | LSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALY NSTRDRVAGESAEPEPEPEADYYAKEVTRVLMVETHNEIYDKFKQSTHSIYM FFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNR LLAPSDSPEWLSFDVTGVVRQWLSRGGEIEGFRLSAHCSCDSRDNTLQVDIN GFTTGRRGDLATIHGMNRPFLLLMATPLERAQHLQSSRHRRAL |
| Product Declaration | 该产品仅供科研使用，不可直接用于人体或注射。 |
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