Recombinant Human CD142/F3 Protein, C-His tag
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产品编号
KMH318
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别名
组织因子, TF, TFA, CD142, coagulation factor III, tissue factor
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规格
- 20ug
- 50ug
- 100ug
- 200ug
- 1mg
产品介绍
| Catalog Number | KMH318 |
| Alias | 组织因子, TF, TFA, CD142, coagulation factor III, tissue factor |
| Size | 20ug, 50ug, 100ug, 200ug, 1mg |
| Product Description | KMH318 Recombinant Human CD142/F3 Protein, C-His tag contains the human F3(GTTNTVAAYNLTWKSTNFKTILEWEPKPVNQVYTVQISTKSGDWKSKCFYTTDTECDLTDEIVKDVKQTYLARVFSYPAGNVESTGSAGEPLYENSPEFTPYLETNLGQPTIQSFEQVGTKVNVTVEDERTLVRRNNTFLSLRDVFGKDLIYTLYYWKSSSSGKKTAKTNTNEFLIDVDKGENYCFSVQAVIPSRTVNRKSTDSPVECMGQEKGEFRE) was expressed in Mammalian cells. |
| Molecular Name | F3 |
| Product Introduction | F3 (KMH318) 作为凝血级联反应的启动因子，参与血液凝固过程。 |
| Molecular Weight | 37-45 kDa |
| Expression System | HEK293F Cells |
| Species | Human |
| Concentration | 联系销售经理获得最新批次的浓度 |
| Purity | ≥95% |
| SDS-PAGE |  |
| Purification | Affinity Purification |
| Uniprot ID | P13726 |
| Storage Condition | Store the product under sterile conditions after opening at -80℃ for 12 months. Store the lyophilized powder at -20℃. Avoid repeated freeze-thaw cycles. |
| Formulation | PBS |
| Shipping Condition | This product is shipped on ice packs or dry ice. |
| Background | Tissue factor is a cell-surface protein of endothelial cells and monocytes involved in coagulation and inflammation. It is the primary cellular initiator of coagulation cascade, and functions as receptor and cofactor for coagulation Factor VII and VIIa.From the rabbit gene using a T-7 cell expression system; purified predominantly to a 45 kD protein, comprising the extracellular and transmembrane domains of the native molecule, plus an amino terminal leader peptide sequence required for processing and purification. |
| Endotoxin | < 0.1 EU per ug |
| Protein Family | CD molecule |
| Biological Activity | Measured by its ability to activate Coagulation Factor VII in cleaving a fluorogenic peptide substrate BocVPR-AMC. The AC50 is <24.0 μg/mL. |
| Product Declaration | 该产品仅供科研使用，不可直接用于人体或注射。 |
FAQ
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可以在293F细胞中共表达多种蛋白吗？
是的，293F细胞可以共表达多种蛋白，但可能需要优化共转染条件（如质粒比例、转染条件）以获得高表达水平。
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在293F蛋白表达中常见的问题有哪些？
常见问题包括低蛋白产量、蛋白折叠不良、聚集和蛋白降解。通过优化转染条件和蛋白纯化方案可以解决这些问题。
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- 手机：13116038708
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