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重组人R-脊椎蛋白1, His标签|Recombinant Human RSPO1 Protein, His Tag

重组人R-脊椎蛋白1, His标签|Recombinant Human RSPO1 Protein, His Tag

产品编号

KMH1655

别名

R-促进因子1,R-脊椎蛋白1,RSPO1,R-spondin 1,RSPO,CRISTIN3

规格
  • 20ug
  • 50ug
  • 100ug
  • 200ug
  • 1mg
产品介绍
Catalog NumberKMH1655
AliasR-促进因子1,R-脊椎蛋白1,RSPO1,R-spondin 1,RSPO,CRISTIN3
Size20ug, 50ug, 100ug, 200ug, 1mg
Molecular NameRSPO1
Product IntroductionR-脊椎蛋白1是一种分泌蛋白,通过特异性增强Wnt/β-catenin信号通路的活性,从而促进细胞的增殖与分化。
Molecular Weight25.63 kDa
Expression SystemMammalian cells
SpeciesHuman
Concentration联系销售经理获得最新批次的浓度
Purity≥90% as determined by SDS-PAGE
PurificationAffinity Purification
Storage ConditionStore the product under sterile conditions after opening at -20℃ to -80℃ for 12 months. Avoid repeated freeze-thaw cycles.
FormulationPBS
Shipping ConditionThis product is shipped on ice packs or dry ice.
BackgroundR-Spondin-1 (Rspo-1) belongs to the (Rspo) family of Wnt modulators. Currently, the family consists of four structurally related secreted ligands (Rspo 1-4), all containing the furin-like and thrombospondin structural domains. Rspo-1 is expressed in certain areas of the developing central nervous system, as well as in the adrenal glands, ovary, testis, thyroid, and trachea. Rspo can interact with the Frizzled/LRP6 receptor complex in a manner that stimulates the Wnt/beta-catenin signaling pathway.
Endotoxin< 1.0 EU per ug of the protein as determined by the LAL method.
Protein FamilyOther protein
Product Declaration该产品仅供科研使用,不可直接用于人体或注射。
FAQ
  • 如何避免重组蛋白降解?

    可在纯化过程中加入蛋白酶抑制剂、低温操作,优化储存条件(如分装冻存于-80°C),并避免反复冻融。某些情况下,可选用蛋白酶缺陷型宿主菌减少降解风险。

  • 如何检测重组蛋白的表达和纯度?

    常用SDS-PAGE、Western Blot检测表达,BCA/Bradford法测浓度,HPLC或质谱分析纯度,生物活性实验(如ELISA、酶活检测)验证功能。

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