Recombinant Human KLKB1 Protein, C-His tag
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产品编号
KMH1311
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别名
激肽原激酶B1, PKK, PPK, KLK3, PKKD, kallikrein B1
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规格
- 20ug
- 50ug
- 100ug
- 200ug
- 1mg
产品介绍
| Alias | 激肽原激酶B1, PKK, PPK, KLK3, PKKD, kallikrein B1 |
| Catalog Number | KMH1311 |
| Molecular Name | KLKB1 |
| Molecular Weight | 80 kDa |
| Size | 20ug, 50ug, 100ug, 200ug, 1mg |
| Concentration | 联系销售经理获得最新批次的浓度 |
| Purity | ≥90% |
| SDS-PAGE |  |
| Product Description | KMH1311 Recombinant Human KLKB1 Protein, C-His tag contains the human KLKB1( 20-638 aa) was expressed in Mammalian cells. |
| Storage Condition | Store the product under sterile conditions after opening at -80℃ for 12 months. Store the lyophilized powder at -20℃. Avoid repeated freeze-thaw cycles. |
| Formulation | PBS |
| Shipping Condition | This product is shipped on ice packs or dry ice. |
| Background | KLKB1 is a 638 amino acids protein that belongs to the peptidase S1 family and plasma kallikrein subfamily. It contains a peptidase S1 domain and four apple domains. The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin. It participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation.Plasma prekallikrein deficiency causes a prolonged activated partial thromboplastin time in patients. |
| Species | Human |
| Protein Family | Other protein |
| Purification | Affinity Purification |
| Endotoxin | < 0.1 EU per ug |
| Biological Activity | Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7- amido-4- methylcoumarin (PFR-AMC). The specific activity is >1,000 pmol/min/µg, as measured under the described conditions. |
| Uniprot ID | P03952 |
| Product Declaration | 该产品仅供科研使用，不可直接用于人体或注射。 |
| Display Peptides | HEK293F Cells |
FAQ
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问：可以在293F细胞中共表达多种蛋白吗？ 答： 是的，293F细胞可以共表达多种蛋白，但可能需要优化共转染条件（如质粒比例、转染条件）以获得高表达水平。 问：在293F蛋白表达中常见的问题有哪些？ 答： 常见问题包括低蛋白产量、蛋白折叠不良、聚集和蛋白降解。通过优化转染条件和蛋白纯化方案可以解决这些问题。
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