| Alias | Raf-1 原癌基因, Human RAF1 Protein, His Tag, GST Tag, RAF1 |
| Catalog Number | KMP2686 |
| Product Description | The Human RAF1 Protein(KMP2686) is produced in Insect Cells. The target gene encoding the human RAF1 (NP_002871.1) (Ser306-Phe648) is expressed with a N-terminal GST tagged polyhistidine tag at the N-terminus. |
| Molecular Name | RAF1 |
| Species | Human |
| Host | Insect Cells |
| Size | 50ug, 100ug, 200ug |
| Purity | >70% as determined by SDS-PAGE |
| Purification | Affinity purification |
| Endotoxin | <1.0 EU/ug determined by the LAL method |
| Formulation | 20mM Tris, 500mM NaCl, 10% Glycerol, 0.5mM TCEP, 2mM GSH, pH8.0 |
| Background | RAF1 gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. |
| Storage Condition | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
| Shipping Condition | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice. |
| Molecular Weight | 66.9 kDa |
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