Recombinant E. coli Mug Protein, No Tag
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产品编号
PO143
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别名
ECK3058, ygjF, stationary phase mismatch/uracil DNA glycosylase
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规格
- 50ug
- 100ug
Alias | ECK3058, ygjF, stationary phase mismatch/uracil DNA glycosylase |
Catalog Number | PO143 |
Molecular Name | mug |
Species | E. coli (strain K12) |
Host | E. coli |
Size | 50ug, 100ug |
Protein Family | Other protein |
Purity | ≥90% as determined by SDS-PAGE |
Purification | Affinity Purification |
Endotoxin | Please contact our customer service for more information. |
Formulation | PBS |
Background | G/U mismatch-specific DNA glycosylase, xanthine DNA glycosylase, also known as mug, belongs to the TDG/mug DNA glycosylase family. It has been proposed that the Mug protein excises 3, N4-ethenocytosine and removes the uracil base from mismatches in the order of U:G>U:A, although the biological role remains unclear. The enzyme Uracil-N-Glycosylase removes uracil from the DNA leaving an AP site. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired base. The complementary strand guanine functions in substrate recognition. Recombinant E.coli mug protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
Storage Condition | Store the product under sterile conditions after opening at -20℃ to -80℃ for 12 months. Avoid repeated freeze-thaw cycles. |
Shipping Condition | This product is shipped on ice packs or dry ice. |
Molecular Weight | 18.59 kDa |
Note | 仅供科研使用 |