Human CD73 Protein, Fc Tag_天津卡梅德科技发展有限公司

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Human CD73 Protein, Fc Tag

Human CD73 Protein, Fc Tag

产品编号

KMP2245

别名

胞外-5′-核苷酸酶, 5'-Nucleotidase, NT5E

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	胞外-5′-核苷酸酶, 5'-Nucleotidase, NT5E
Catalog Number	KMP2245
Product Description	The Human CD73 Protein(KMP2245) is produced in HEK293 Cells and the target gene encoding Trp27-Lys547 is expressed with a Fc tag at the C-terminus.
Molecular Name	CD73
Species	Human
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	20mM TrisHCl, 120mM NaCl, 4mM CaCl2, 20% Glycerol, pH7.5
Background	CD73 is a glycosyl phosphatidylinositol(GPI) anchored membrane protein that belongs to the 5'-nucleotidase family. CD73 is an ecto 5'Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'Nucleotidase activity. Defects in NT5E are the cause of calcification of joints and arteries(CAJA). The recombinant CD73 lacking GPI anchor is secreted as a monomer.
SDS-PAGE
Predicted Molecular Weight	57.79 kDa
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Uniprot ID	P21589
References	1.Biochem. J. 441:131-141 (2012) 2.ChemBioChem 13:2384-2391 (2012) 3.Structure 20:2161-2173 (2012) 4.PLoS ONE 9:E98568-E98568 (2014) 5.Purinergic Signal. 17:693-704 (2021)
Function	Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate (PubMed:21933152, PubMed:34403084, PubMed:24887587, PubMed:22997138, PubMed:23142347). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (PubMed:34403084). Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (PubMed:21933152, PubMed:34403084, PubMed:24887587, PubMed:22997138, PubMed:23142347).

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