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Human eIF5A Protein, His Tag

Human eIF5A Protein, His Tag

产品编号

KMP3184

别名

真核翻译起始因子 5A, Human eIF5A Protein, His Tag, eIF5A

规格
  • 50ug
  • 100ug
  • 200ug
产品介绍
Alias真核翻译起始因子 5A, Human eIF5A Protein, His Tag, eIF5A
Catalog NumberKMP3184
Product DescriptionThe Human eIF5A Protein(KMP3184) is produced in E. coli. The target gene encoding the human EIF5A (NP_001961.1) (Met1-Lys154) is expressed with a polyhistidine tag at the N-terminus.
Molecular NameeIF5A
SpeciesHuman
HostE. coli
Size50ug, 100ug, 200ug
Purity>85% as determined by SDS-PAGE
PurificationAffinity purification
Endotoxin<1.0 EU/ug determined by the LAL method
FormulationPBS, pH7.4
BackgroundEIF-5A, also known as EIF5, functions in start site selection as a GTPase accelerating protein (GAP) for the eukaryotic translation initiation factor (eIF) 2•GTP•tRNA ternary complex within the ribosome-bound pre-initiation complex. In protein synthesis initiation, eIF2 functions in its GTP-bound state to deliver initiator methionyl-tRNA to the small ribosomal subunit and is necessary for protein synthesis in all cells. EIF-5A stabilizes the binding of GDP to eIF2 and is therefore a bi-functional protein that acts as a GDP dissociation inhibitor (GDI). EIF-5A also interacts with eIF1 and eIF3 and binds the eIF2-GTP/Met-tRNA ternary complex along with the 40S ribosome subunit.
Storage ConditionAliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping ConditionIn general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.
Molecular Weight18.9 kDa

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