Human eIF5A Protein, His Tag
-
产品编号
KMP3184
-
别名
真核翻译起始因子 5A, Human eIF5A Protein, His Tag, eIF5A
-
规格
- 50ug
- 100ug
- 200ug
Alias | 真核翻译起始因子 5A, Human eIF5A Protein, His Tag, eIF5A |
Catalog Number | KMP3184 |
Product Description | The Human eIF5A Protein(KMP3184) is produced in E. coli. The target gene encoding the human EIF5A (NP_001961.1) (Met1-Lys154) is expressed with a polyhistidine tag at the N-terminus. |
Molecular Name | eIF5A |
Species | Human |
Host | E. coli |
Size | 50ug, 100ug, 200ug |
Purity | >85% as determined by SDS-PAGE |
Purification | Affinity purification |
Endotoxin | <1.0 EU/ug determined by the LAL method |
Formulation | PBS, pH7.4 |
Background | EIF-5A, also known as EIF5, functions in start site selection as a GTPase accelerating protein (GAP) for the eukaryotic translation initiation factor (eIF) 2•GTP•tRNA ternary complex within the ribosome-bound pre-initiation complex. In protein synthesis initiation, eIF2 functions in its GTP-bound state to deliver initiator methionyl-tRNA to the small ribosomal subunit and is necessary for protein synthesis in all cells. EIF-5A stabilizes the binding of GDP to eIF2 and is therefore a bi-functional protein that acts as a GDP dissociation inhibitor (GDI). EIF-5A also interacts with eIF1 and eIF3 and binds the eIF2-GTP/Met-tRNA ternary complex along with the 40S ribosome subunit. |
Storage Condition | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
Shipping Condition | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice. |
Molecular Weight | 18.9 kDa |