Human KRAS Protein, His Tag_天津卡梅德科技发展有限公司

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Human KRAS Protein, His Tag

Human KRAS Protein, His Tag

产品编号

KMP2999

别名

Kirsten大鼠肉瘤病毒癌基因同源物, Human KRAS Protein, His Tag, KRAS

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	Kirsten大鼠肉瘤病毒癌基因同源物, Human KRAS Protein, His Tag, KRAS
Catalog Number	KMP2999
Product Description	The Human KRAS Protein(KMP2999) is produced in E. coli. The target gene encoding the human KRAS (P01116-2) (Thr2-Cys185, with natural variant Gly 12 Cys) is expressed with a polyhistidine tag at the N-terminus.
Molecular Name	KRAS
Species	Human
Host	E. coli
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	25mM Tris, 100mM Glycerolcine, 10% Glycerol, pH7.5
Background	K-Ras belongs to the small GTPase superfamily, Ras family. Like other members of the Ras family, K-Ras is a GTPase and is an early player in many signal transduction pathways. It is usually tethered to cell membranes because of the presence of an isoprenyl group on its C-terminus. K-Ras functions as a molecular on/off switch. Once it is turned on it recruits and activates proteins necessary for the propagation of growth factor and other receptors' signal, such as c-Raf and PI 3-kinase. It binds to GTP in the active state and possesses an intrinsic enzymatic activity that cleaves the terminal phosphate of the nucleotide converting it to GDP. Upon conversion of GTP to GDP, K-Ras is turned off. The rate of conversion is usually slow but can be sped up dramatically by an accessory protein of the GTPase activating protein class, for example, RasGAP. In turn, K-Ras can bind to proteins of the Guanine Nucleotide Exchange Factor class, for example, SOS1, which forces the release of bound nucleotide. Subsequently, K-Ras binds GTP present in the cytosol and the GEF is released from ras-GTP. Besides essential function in normal tissue signaling, the mutation of a K-Ras gene is an essential step in the development of many cancers. Several germline K-Ras mutations are associated with Noonan syndrome and Cardio-Facio-Cutaneous syndrome. Somatic K-Ras mutations are found at high rates in Leukemias, colon cancer, pancreatic cancer, and lung cancer.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.
Molecular Weight	22 kDa

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