Human CTNNB1 Protein, His Tag, GST Tag_天津卡梅德科技发展有限公司

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Human CTNNB1 Protein, His Tag, GST Tag

Human CTNNB1 Protein, His Tag, GST Tag

产品编号

KMP2880

别名

连环蛋白 1, Human CTNNB1 Protein, His Tag, GST Tag, CTNNB1

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	连环蛋白 1, Human CTNNB1 Protein, His Tag, GST Tag, CTNNB1
Catalog Number	KMP2880
Product Description	The Human CTNNB1 Protein(KMP2880) is produced in Insect Cells. The target gene encoding the human CTNNB1 (P35222-1) (Met 1-Leu 781) is fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Molecular Name	CTNNB1
Species	Human
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>85% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	50mM Tris, 150mM NaCl, 25% Glycerol, 0.1mM EDTA, 1mM TCEP, 0.4mM PMSF, 0.5mM GSH, pH8.0
Background	beta-Catenin, also known as CTNNB1, is a member of the armadillo family of proteins. These proteins have multiple copies of the so-called armadillo repeat domain, which is specialized for protein-protein binding. It is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. CTNNB1 also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, beta-Catenin binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Defects in beta-Catenin can cause colorectal cancer, pilomatrixoma (PTR), medulloblastoma, and ovarian cancer. CTNNB1 is a key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, it forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, beta-Catenin is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. CTNNB1 is involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.
Molecular Weight	113 kDa

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