Human IL-35 Protein, Fc-Flag Tag, Fc-His Tag

产品编号

KMP3559

别名

白细胞介素35, Human IL-35 Protein, Fc-Flag Tag, Fc-His Tag, IL-35

目录价格

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规格

50ug
100ug
200ug

DATASHEET COA

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产品介绍

Alias	白细胞介素35, Human IL-35 Protein, Fc-Flag Tag, Fc-His Tag, IL-35
Catalog Number	KMP3559
Product Description	The Human IL-35 Protein(KMP3559) is produced in HEK293 Cells. The target gene encoding the p35 subunit of human IL12, termed as IL12A (P29459-1) (Met1-Ser219) is fused with a C-terminal Fc region of human IgG1 tag followed by a FLAG tag, constructed the plasmid 1; The target gene encoding the human IL27B (NP_005746.2) (Met1-Lys229) is fused with a C-terminal Fc region of human IgG1 tag followed by a polyhistidine tag, constructed the plasmid 2. The two plasmids are co-expressed and the heterodimer is purified.
Molecular Name	IL-35
Species	Human
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Protein Family	Other protein
Purity	>90% as determined by SDS-PAGE
Concentration	联系销售经理获得最新批次的浓度
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	IL12A is a subunit of a cytokine that acts on T and natural killer cells and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. IL12A, together with IL27B, form a disulfide-linked heterodimer: IL12A&IL27B. IL12A&IL27B is required for the T-cell-independent induction of IFN-gamma and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of IL12A&IL27B in innate immunity.
Application	WB, ELISA
Recommended Dilution	ELISA: 1:100-1:1000
Cross-reactivity	No cross-reactivity with other antigens or proteins.
Predicted Molecular Weight	102.1 kDa
Predicted N Terminus	Met
Reconstitution	Not applicable. The product is supplied as a ready-to-use liquid formulation.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Remarks	收到产品后请立即按保存条件储存；使用前短暂离心，使内容物集中于管底；避免反复冻融。
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.
Stability	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Molecular Weight	102.1 kDa
Cross-reactivity	No cross-reactivity with other antigens or proteins.
Note	仅供科研使用
ELISA IC50(ppb)	≥ 1:1000
Function	IL12A is a subunit of a cytokine that acts on T and natural killer cells and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. IL12A, together with IL27B, form a disulfide-linked heterodimer: IL12A&IL27B. IL12A&IL27B is required for the T-cell-independent induction of IFN-gamma and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of IL12A&IL27B in innate immunity.
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。
Temperature Range	-20℃ ~ -80℃
Solubility	产品为即用型液体，无需复溶。
Appearance	Liquid
Product Full Name	Human IL-35 Protein, Fc-Flag Tag
Preservative Solution	PBS, pH7.4
Usage Method	冻干粉请按“复溶”说明用无菌去离子水复溶后使用，液体产品可直接使用；具体工作浓度请参考“推荐稀释比例”。
Target	IL-35