| Alias | 干扰素α2a, INFα2a, Interferon alpha 2a, IFNA2, IFNA2a, human IFNA2a protein, Interferon alpha 2 protein, IFNA2 |
| Catalog Number | KMH1256 |
| Molecular Name | Interferon alpha 2, IFNA2 |
| Species | Human |
| Host | E.coli |
| Size | 50ug, 500ug, 1mg |
| Protein Family | Other protein |
| Purity | > 95% as determined by Tris-Bis PAGE, > 95% as determined by HPLC |
| Concentration | 联系销售经理获得最新批次的浓度 |
| Purification | Affinity Purification |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
| Background | At least 23 different variants of IFN-α are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-α subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-α subtypes differ in their sequences by only one or two positions. Naturally occurring variants also include proteins that are truncated by 10 amino acids at the carboxyl-terminal end. |
| Application | SDS-PAGE, WB, ELISA |
| Recommended Dilution | ELISA: 1:100-1:1000 |
| Tag | No Tag |
| Biological Activity | Measured in antiviral assay using A549 human lung cancer cells infected with vesicular stomatitisvirus (VSV) The ED50 for this effect is 5 ng/mL. |
| Cross-reactivity | No cross-reactivity with other antigens or proteins. |
| Predicted Molecular Weight | 19.4 kDa |
| Predicted N Terminus | Cys 24 |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage Condition | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Remarks | 收到产品后请立即按保存条件储存；使用前短暂离心，使内容物集中于管底；避免反复冻融。 |
| Shipping Condition | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
| Stability | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Sequence | CDLPQTHSLGSRRTLMLLAQMRRISLFSCLKDRHDFGFPQEEFGNQFQKAETI PVLHEMIQQIFNLFSTKDSSAAWDETLLDKFYTELYQQLNDLEACVIQGVGVT ETPLMKEDSILAVRKYFQRITLYLKEKKYSPCAWEVVRAEIMRSFSLSTNLQE SLRSKE |
| Molecular Weight | The protein has a predicted MW of 19.4 kDa. the protein migrates to 17 kDa in SDS-PAGE under reducing conditions. |
| Cross-reactivity | No cross-reactivity with other antigens or proteins. |
| Uniprot ID | P01563 |
| Note | 仅供科研使用 |
| ELISA IC50(ppb) | ≥ 1:1000 |
| Function | At least 23 different variants of IFN-α are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-α subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-α subtypes differ in their sequences by only one or two positions. Naturally occurring variants also include proteins that are truncated by 10 amino acids at the carboxyl-terminal end. |
| Product Declaration | 该产品仅供科研使用，不可直接用于人体或注射。 |
| Temperature Range | -20℃ ~ -80℃ |
| Appearance | Lyophilized powder |
| Product Full Name | Recombinant Human IFN-α2b/IFNA2 Protein |
| Preservative Solution | 20mM PB, 150mM NaCl, pH 7.2 |
| Usage Method | 冻干粉请按“复溶”说明用无菌去离子水复溶后使用，液体产品可直接使用；具体工作浓度请参考“推荐稀释比例”。 |
| Target | Interferon alpha 2, IFNA2 |
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