Mouse CLEC4F Protein, hFc Tag
-
产品编号
KMP3880
-
别名
C型凝集素结构域家族4成员F, CLEC4F Protein, Mouse CLEC4F Protein, hFc Tag
-
目录价格
联系客服获取报价RMB
-
规格
- 50ug
- 100ug
- 200ug
产品介绍
| Alias | C型凝集素结构域家族4成员F, CLEC4F Protein, Mouse CLEC4F Protein, hFc Tag |
| Catalog Number | KMP3880 |
| Product Description | The Mouse CLEC4F Protein(KMP3880) is produced in HEK293 Cells. A DNA sequence encoding the Mouse Clec4f (NP_058031.2) (Arg70-Gly548) is expressed with the Fc region of human IgG1 at the N-terminus. |
| Molecular Name | CLEC4F |
| Species | Mouse |
| Host | HEK293 Cells |
| Size | 50ug, 100ug, 200ug |
| Protein Family | Other protein |
| Purity | >85% as determined by SDS-PAGE |
| Concentration | 联系销售经理获得最新批次的浓度 |
| Purification | Affinity purification |
| Endotoxin | <1.0 EU/ug determined by the LAL method |
| Formulation | PBS, pH7.4 |
| Background | CLEC4F, a member of C-type lectins, was firstly purified from rat liver extract with high binding affinity to fucose, galactose and N-acetylgalactosamine, and un-sialylated glucosphingolipids with GalNAc or Gal terminus. However, the biological functions of CLEC4F have not been elucidated. Histochemical staining showed that mouse CLEC4F(mCLEC4F) is only expressed on F4/8+ cells localized in liver, and is undetectable in bone marrow, spleen, lymph nodes, or other tissues in adult mice. However, mCLEC4F is detected in the liver of embryonic day 11.5 (E11.5), which is 1.5 day earlier than the formation of liver (E1) and is 3.5 day earlier than the formation of bone marrow (E15-16). Moreover, recombinant mCLEC4F.Fc binds to alpha-galactoceramide in a Ca++-dependent manner, and both galactose and ceramide can partially inhibit CLEC4F.Fc binding to alpha-galactoceramide. Interestingly, mCLEC4F-deficient (mCEC4F k/o) mice produced far less cytokines than wild type littermates after intravenous injection ofalpha-galactoceramide. This suggests that mCLEC4F is not only a specific marker for Kupffer cells, but is also critical for the presentation of glycolipid antigen to NKT cells. |
| Application | WB, ELISA |
| Recommended Dilution | ELISA: 1:100-1:1000 |
| Cross-reactivity | No cross-reactivity with other antigens or proteins. |
| Predicted Molecular Weight | 82.1 kDa |
| Predicted N Terminus | Arg 70 |
| Reconstitution | Not applicable. The product is supplied as a ready-to-use liquid formulation. |
| Storage Condition | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
| Remarks | 收到产品后请立即按保存条件储存；使用前短暂离心，使内容物集中于管底；避免反复冻融。 |
| Shipping Condition | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice. |
| Stability | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Molecular Weight | 82.1 kDa |
| Cross-reactivity | No cross-reactivity with other antigens or proteins. |
| Uniprot ID | D18063 |
| Note | 仅供科研使用 |
| ELISA IC50(ppb) | ≥ 1:1000 |
| Function | CLEC4F, a member of C-type lectins, was firstly purified from rat liver extract with high binding affinity to fucose, galactose and N-acetylgalactosamine, and un-sialylated glucosphingolipids with GalNAc or Gal terminus. However, the biological functions of CLEC4F have not been elucidated. Histochemical staining showed that mouse CLEC4F(mCLEC4F) is only expressed on F4/8+ cells localized in liver, and is undetectable in bone marrow, spleen, lymph nodes, or other tissues in adult mice. However, mCLEC4F is detected in the liver of embryonic day 11.5 (E11.5), which is 1.5 day earlier than the formation of liver (E1) and is 3.5 day earlier than the formation of bone marrow (E15-16). Moreover, recombinant mCLEC4F.Fc binds to alpha-galactoceramide in a Ca++-dependent manner, and both galactose and ceramide can partially inhibit CLEC4F.Fc binding to alpha-galactoceramide. Interestingly, mCLEC4F-deficient (mCEC4F k/o) mice produced far less cytokines than wild type littermates after intravenous injection ofalpha-galactoceramide. This suggests that mCLEC4F is not only a specific marker for Kupffer cells, but is also critical for the presentation of glycolipid antigen to NKT cells. |
| Product Declaration | 该产品仅供科研使用，不可直接用于人体或注射。 |
| Temperature Range | -20℃ ~ -80℃ |
| Solubility | 产品为即用型液体，无需复溶。 |
| Appearance | Liquid |
| Product Full Name | Mouse CLEC4F Protein |
| Preservative Solution | PBS, pH7.4 |
| Usage Method | 冻干粉请按“复溶”说明用无菌去离子水复溶后使用，液体产品可直接使用；具体工作浓度请参考“推荐稀释比例”。 |
| Target | CLEC4F |
- 联系我们
- 电话：400-621-6806
- 手机：13116038708（微信）
- 邮箱：info@kmdbioscience.com
- 地址：天津市武清开发区和畅路6号10号厂房108室
-
销售微信
-
官方公众号
-
400-621-6806 -
3599163699 -
周一至周五 9:00-18:00 -
-
0
-
