| Alias | TGF Beta 1和GARP的异二聚体蛋白, Human TGF Beta 1 & GARP Heterotrimer Protein, His Tag, TGF Beta 1 & GARP Heterotrimer |
| Catalog Number | KMP3566 |
| Product Description | The Human TGF Beta 1 & GARP Heterotrimer Protein(KMP3566) is produced in HEK293 Cells. The target gene encoding the human TGFB1 (P01137)(Met1-Ser390) is expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 1; The target gene encoding the human GARP (Q14392) (Met1-Asn627) is expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids are co-expressed and the TGF Beta 1 & GARP Heterotrimer is purified. |
| Molecular Name | TGF Beta 1 & GARP Heterotrimer |
| Species | Human |
| Host | HEK293 Cells |
| Size | 50ug, 100ug, 200ug |
| Protein Family | Other protein |
| Purity | >95% as determined by SDS-PAGE |
| Concentration | 联系销售经理获得最新批次的浓度 |
| Purification | Affinity purification |
| Endotoxin | <1.0 EU/ug determined by the LAL method |
| Formulation | PBS, pH7.4 |
| Background | TGFB1 (Transforming Growth Factor Beta 1) is a Protein Coding gene. Diseases associated with TGFB1 include Camurati-Engelmann Disease and Inflammatory Bowel Disease, Immunodeficiency, And Encephalopathy. Among its related pathways are Apoptotic Pathways in Synovial Fibroblasts and GPCR Pathway. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and enzyme binding. An important paralog of this gene is TGFB3. GARP (LRRC32) is a type I transmembrane protein in which the extracellular region has two sets of 10 LRRs (leucine-rich repeats) regions connected by proline-rich structural domains (proline domains). In the inactive state (Latent) TGF-β forms a disulfide bond with GARP via a cysteine on its LAP. |
| Application | WB, ELISA |
| Recommended Dilution | ELISA: 1:100-1:1000 |
| Cross-reactivity | No cross-reactivity with other antigens or proteins. |
| Predicted Molecular Weight | 108.72 kDa |
| Predicted N Terminus | Met |
| Reconstitution | Not applicable. The product is supplied as a ready-to-use liquid formulation. |
| Storage Condition | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
| Remarks | 收到产品后请立即按保存条件储存；使用前短暂离心，使内容物集中于管底；避免反复冻融。 |
| Shipping Condition | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice. |
| Stability | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Molecular Weight | 108.72 kDa |
| Cross-reactivity | No cross-reactivity with other antigens or proteins. |
| Note | 仅供科研使用 |
| ELISA IC50(ppb) | ≥ 1:1000 |
| Function | TGFB1 (Transforming Growth Factor Beta 1) is a Protein Coding gene. Diseases associated with TGFB1 include Camurati-Engelmann Disease and Inflammatory Bowel Disease, Immunodeficiency, And Encephalopathy. Among its related pathways are Apoptotic Pathways in Synovial Fibroblasts and GPCR Pathway. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and enzyme binding. An important paralog of this gene is TGFB3. GARP (LRRC32) is a type I transmembrane protein in which the extracellular region has two sets of 10 LRRs (leucine-rich repeats) regions connected by proline-rich structural domains (proline domains). In the inactive state (Latent) TGF-β forms a disulfide bond with GARP via a cysteine on its LAP. |
| Product Declaration | 该产品仅供科研使用，不可直接用于人体或注射。 |
| Temperature Range | -20℃ ~ -80℃ |
| Solubility | 产品为即用型液体，无需复溶。 |
| Appearance | Liquid |
| Product Full Name | Human TGF Beta 1 & GARP Heterotrimer Protein |
| Preservative Solution | PBS, pH7.4 |
| Usage Method | 冻干粉请按“复溶”说明用无菌去离子水复溶后使用，液体产品可直接使用；具体工作浓度请参考“推荐稀释比例”。 |
| Target | TGF Beta 1 & GARP Heterotrimer |
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