Human IL18 Protein
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产品编号
KMPH1232
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别名
白介素-18, IL18, interleukin 18, IGIF, IL-18, IL-1g, IL1F4
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目录价格
联系客服获取报价RMB
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规格
- 10ug
- 100ug
- 500ug
- 1mg
产品介绍
| Alias | 白介素-18, IL18, interleukin 18, IGIF, IL-18, IL-1g, IL1F4 |
| Catalog Number | KMPH1232 |
| Molecular Name | Interleukin 18 IL 18 |
| Species | Human |
| Host | E.coli |
| Size | 10ug, 100ug, 500ug, 1mg |
| Protein Family | CD molecule |
| Purity | > 95% as determined by Tris-Bis PAGE, > 95% as determined by HPLC |
| Concentration | 联系销售经理获得最新批次的浓度 |
| Purification | Affinity Purification |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Formulation | Lyophilized from 0.22um filtered solution in 20mM PB, 250mM NaCl, 50mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Background | Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naïve T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation. |
| Application | SDS-PAGE, WB, ELISA |
| Recommended Dilution | ELISA: 1:100-1:1000 |
| Tag | No Tag |
| Biological Activity | Immobilized Human IL-18 at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-18BP, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA (QC Test). |
| Cross-reactivity | No cross-reactivity with other antigens or proteins. |
| Predicted Molecular Weight | 18.2 kDa |
| Predicted N Terminus | Tyr 37 |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage Condition | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Remarks | 收到产品后请立即按保存条件储存；使用前短暂离心，使内容物集中于管底；避免反复冻融。 |
| Shipping Condition | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
| Stability | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Sequence | YFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISM YKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFF QRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTV QNED |
| Molecular Weight | The protein has a predicted MW of 18.2 kDa same as Tris-Bis PAGE result. |
| Cross-reactivity | No cross-reactivity with other antigens or proteins. |
| Uniprot ID | Q14116-1 |
| Note | 仅供科研使用 |
| ELISA IC50(ppb) | ≥ 1:1000 |
| Function | Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naïve T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation. |
| Product Declaration | 该产品仅供科研使用，不可直接用于人体或注射。 |
| Temperature Range | -20℃ ~ -80℃ |
| Solubility | 复溶后易溶于无菌去离子水或PBS缓冲液（pH 7.4）。 |
| Appearance | Lyophilized powder |
| Product Full Name | Human IL18 Protein |
| Preservative Solution | 0.22um filtered solution in 20mM PB, 250mM NaCl, 50mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization |
| Usage Method | 冻干粉请按“复溶”说明用无菌去离子水复溶后使用，液体产品可直接使用；具体工作浓度请参考“推荐稀释比例”。 |
| Target | Interleukin 18 IL 18 |
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