Canine SDF-1 Protein

产品编号

KMP3603

别名

基质细胞衍生因子-1, SDF-1 Protein, Canine SDF-1 Protein

目录价格

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规格

50ug
100ug
200ug

DATASHEET COA

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产品介绍

Alias	基质细胞衍生因子-1, SDF-1 Protein, Canine SDF-1 Protein
Catalog Number	KMP3603
Product Description	The Canine SDF-1 Protein(KMP3603) is produced in E. coli. A DNA sequence encoding the Canine CXCL12 (NP_001121569.1) (Lys22-Met93) is expressed with a N-terminal Met.
Molecular Name	SDF-1
Species	Canine
Host	E. coli
Size	50ug, 100ug, 200ug
Protein Family	CD molecule
Purity	>95% as determined by SDS-PAGE
Concentration	联系销售经理获得最新批次的浓度
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	50mM Tris, 500mM NaCl, pH8.0
Background	The human stromal cell-derived factor-1 (SDF1), also known as CXCL12, is a small (8 kDa) cytokine highly conserved chemotactic cytokine belonging to the large family of CXC chemokines. SDF1 is expressed in two isoforms from a single gene that encodes two splice variants, SDF1α and SDF1β, which are identical except for the four residues present in the C-terminus of SDF1β but absent from SDF1α. The chemokine CXCL12 [stromal cell-derived factor-1 (SDF-1)] binds primarily to CXC receptor 4 (CXCR4; CD184). The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription. CXCL12 and CXCR4 that have been widely characterized in peripheral tissues and delineate their main functions in the CNS. Extensive evidence supports CXCL12 as a key regulator for early development of the CNS. In the mature CNS, CXCL12 modulates neurotransmission, neurotoxicity and neuroglial interactions. CXCL12 has crucial roles in the formation of multiple organ systems during embryogenesis and in the regulation of bone marrow haematopoiesis and immune function in the postnatal organism. Although considered an important factor in normal bone metabolism, recent studies implicate CXCL12 in the pathogenesis of several diseases involving the skeleton, including rheumatoid arthritis and cancers that metastasize to bone. The CXCL12/CXCR4 axis is involved in tumor progression, angiogenesis, metastasis, and survival. Pathologically enhanced CXCL12 signaling may promote the formation of new vessels through recruiting circulating endothelial progenitor cells or directly enhancing the migration/growth of endothelial cells. Therefore, CXCL12 signaling represents an important mechanism that regulates brain tumor angiogenesis/vasculogenesis and may provide potential targets for anti-angiogenic therapy in malignant gliomas.
Application	WB, ELISA
Recommended Dilution	ELISA: 1:100-1:1000
Cross-reactivity	No cross-reactivity with other antigens or proteins.
Predicted Molecular Weight	8.6 kDa
Predicted N Terminus	Lys 22
Reconstitution	Not applicable. The product is supplied as a ready-to-use liquid formulation.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Remarks	收到产品后请立即按保存条件储存；使用前短暂离心，使内容物集中于管底；避免反复冻融。
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.
Stability	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Molecular Weight	8.6 kDa
Cross-reactivity	No cross-reactivity with other antigens or proteins.
Note	仅供科研使用
ELISA IC50(ppb)	≥ 1:1000
Function	The human stromal cell-derived factor-1 (SDF1), also known as CXCL12, is a small (8 kDa) cytokine highly conserved chemotactic cytokine belonging to the large family of CXC chemokines. SDF1 is expressed in two isoforms from a single gene that encodes two splice variants, SDF1α and SDF1β, which are identical except for the four residues present in the C-terminus of SDF1β but absent from SDF1α. The chemokine CXCL12 [stromal cell-derived factor-1 (SDF-1)] binds primarily to CXC receptor 4 (CXCR4; CD184). The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription. CXCL12 and CXCR4 that have been widely characterized in peripheral tissues and delineate their main functions in the CNS. Extensive evidence supports CXCL12 as a key regulator for early development of the CNS. In the mature CNS, CXCL12 modulates neurotransmission, neurotoxicity and neuroglial interactions. CXCL12 has crucial roles in the formation of multiple organ systems during embryogenesis and in the regulation of bone marrow haematopoiesis and immune function in the postnatal organism. Although considered an important factor in normal bone metabolism, recent studies implicate CXCL12 in the pathogenesis of several diseases involving the skeleton, including rheumatoid arthritis and cancers that metastasize to bone. The CXCL12/CXCR4 axis is involved in tumor progression, angiogenesis, metastasis, and survival. Pathologically enhanced CXCL12 signaling may promote the formation of new vessels through recruiting circulating endothelial progenitor cells or directly enhancing the migration/growth of endothelial cells. Therefore, CXCL12 signaling represents an important mechanism that regulates brain tumor angiogenesis/vasculogenesis and may provide potential targets for anti-angiogenic therapy in malignant gliomas.
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。
Temperature Range	-20℃ ~ -80℃
Solubility	产品为即用型液体，无需复溶。
Appearance	Liquid
Product Full Name	Canine SDF-1 Protein
Preservative Solution	50mM Tris, 500mM NaCl, pH8.0
Usage Method	冻干粉请按“复溶”说明用无菌去离子水复溶后使用，液体产品可直接使用；具体工作浓度请参考“推荐稀释比例”。
Target	SDF-1