| Alias | CD3D和CD3E的异二聚体蛋白, Human CD3D & CD3E Heterodimer Protein, Flag Tag, His Tag, CD3D & CD3E Heterodimer |
| Catalog Number | KMP3540 |
| Product Description | The Human CD3D & CD3E Heterodimer Protein(KMP3540) is produced in HEK293 Cells. The target gene encoding the extracellular domain (Met1-Ala105) of human CD3D (P04234) is fused with a flag tag at the C-terminus, constructed the plasmid 1; The target gene encoding the extracellular domain (Met1-Asp126) of human CD3E (P07766) is fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids are co-expressed and the human CD3D/CD3E heterodimer is purified. The purified protein is biotinylated in vitro. |
| Molecular Name | CD3D & CD3E Heterodimer |
| Species | Human |
| Host | HEK293 Cells |
| Size | 50ug, 100ug, 200ug |
| Protein Family | CD molecule |
| Purity | >95% as determined by SDS-PAGE |
| Concentration | 联系销售经理获得最新批次的浓度 |
| Purification | Affinity purification |
| Endotoxin | <1.0 EU/ug determined by the LAL method |
| Formulation | PBS, pH7.4 |
| Background | CD3D (CD3 Delta Subunit Of T-Cell Receptor Complex) is a Protein Coding gene. Diseases associated with CD3D include Immunodeficiency 19 and T-B+ Severe Combined Immunodeficiency Due To Cd3delta/Cd3epsilon/Cd3zeta. Among its related pathways are Immune response NFAT in immune response and TCR Signaling (Qiagen). Gene Ontology (GO) annotations related to this gene include protein heterodimerization activity and transmembrane signaling receptor activity. An important paralog of this gene is CD3G. CD3E (CD3 Epsilon Subunit Of T-Cell Receptor Complex) is a Protein Coding gene. Diseases associated with CD3E include Immunodeficiency 18 and T-B+ Severe Combined Immunodeficiency Due To Cd3delta/Cd3epsilon/Cd3zeta. Among its related pathways are Immune response NFAT in immune response and TCR Signaling (Qiagen). Gene Ontology (GO) annotations related to this gene include protein heterodimerization activity and obsolete signal transducer activity. An important paralog of this gene is CD3G. |
| Application | WB, ELISA |
| Recommended Dilution | ELISA: 1:100-1:1000 |
| Cross-reactivity | No cross-reactivity with other antigens or proteins. |
| Predicted Molecular Weight | 31.05 kDa |
| Predicted N Terminus | Met |
| Reconstitution | Not applicable. The product is supplied as a ready-to-use liquid formulation. |
| Storage Condition | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
| Remarks | 收到产品后请立即按保存条件储存；使用前短暂离心，使内容物集中于管底；避免反复冻融。 |
| Shipping Condition | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice. |
| Stability | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Molecular Weight | 31.05 kDa |
| Cross-reactivity | No cross-reactivity with other antigens or proteins. |
| Note | 仅供科研使用 |
| ELISA IC50(ppb) | ≥ 1:1000 |
| Function | CD3D (CD3 Delta Subunit Of T-Cell Receptor Complex) is a Protein Coding gene. Diseases associated with CD3D include Immunodeficiency 19 and T-B+ Severe Combined Immunodeficiency Due To Cd3delta/Cd3epsilon/Cd3zeta. Among its related pathways are Immune response NFAT in immune response and TCR Signaling (Qiagen). Gene Ontology (GO) annotations related to this gene include protein heterodimerization activity and transmembrane signaling receptor activity. An important paralog of this gene is CD3G. CD3E (CD3 Epsilon Subunit Of T-Cell Receptor Complex) is a Protein Coding gene. Diseases associated with CD3E include Immunodeficiency 18 and T-B+ Severe Combined Immunodeficiency Due To Cd3delta/Cd3epsilon/Cd3zeta. Among its related pathways are Immune response NFAT in immune response and TCR Signaling (Qiagen). Gene Ontology (GO) annotations related to this gene include protein heterodimerization activity and obsolete signal transducer activity. An important paralog of this gene is CD3G. |
| Product Declaration | 该产品仅供科研使用，不可直接用于人体或注射。 |
| Temperature Range | -20℃ ~ -80℃ |
| Solubility | 产品为即用型液体，无需复溶。 |
| Appearance | Liquid |
| Product Full Name | Human CD3D & CD3E Heterodimer Protein, Flag Tag |
| Preservative Solution | PBS, pH7.4 |
| Usage Method | 冻干粉请按“复溶”说明用无菌去离子水复溶后使用，液体产品可直接使用；具体工作浓度请参考“推荐稀释比例”。 |
| Target | CD3D & CD3E Heterodimer |
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