Human CD73 Protein, Fc Tag

产品编号

KMP2245

别名

胞外-5′-核苷酸酶, 5'-Nucleotidase, NT5E

目录价格

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规格

50ug
100ug
200ug

DATASHEET COA

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产品介绍

Alias	胞外-5′-核苷酸酶, 5'-Nucleotidase, NT5E
Catalog Number	KMP2245
Product Description	The Human CD73 Protein(KMP2245) is produced in HEK293 Cells and the target gene encoding Trp27-Lys547 is expressed with a Fc tag at the C-terminus.
Molecular Name	CD73
Species	Human
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Protein Family	CD molecule
Purity	>95% as determined by SDS-PAGE
Concentration	联系销售经理获得最新批次的浓度
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	20mM TrisHCl, 120mM NaCl, 4mM CaCl2, 20% Glycerol, pH7.5
Background	CD73 is a glycosyl phosphatidylinositol(GPI) anchored membrane protein that belongs to the 5'-nucleotidase family. CD73 is an ecto 5'Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'Nucleotidase activity. Defects in NT5E are the cause of calcification of joints and arteries(CAJA). The recombinant CD73 lacking GPI anchor is secreted as a monomer.
Application	WB, ELISA
Recommended Dilution	ELISA: 1:100-1:1000
Cross-reactivity	No cross-reactivity with other antigens or proteins.
Molecular Formula	KMP2245 SDS-PAGE image1.png
SDS-PAGE
Predicted Molecular Weight	57.79 kDa
Predicted N Terminus	Met
Reconstitution	Not applicable. The product is supplied as a ready-to-use liquid formulation.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Remarks	收到产品后请立即按保存条件储存；使用前短暂离心，使内容物集中于管底；避免反复冻融。
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Stability	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Cross-reactivity	No cross-reactivity with other antigens or proteins.
Uniprot ID	P21589
Note	仅供科研使用
ELISA IC50(ppb)	≥ 1:1000
Function	Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate (PubMed:21933152, PubMed:34403084, PubMed:24887587, PubMed:22997138, PubMed:23142347). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (PubMed:34403084). Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (PubMed:21933152, PubMed:34403084, PubMed:24887587, PubMed:22997138, PubMed:23142347).
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。
Temperature Range	-20℃ ~ -80℃
Solubility	产品为即用型液体，无需复溶。
Appearance	Liquid
Product Full Name	Human CD73 Protein
Preservative Solution	20mM TrisHCl, 120mM NaCl, 4mM CaCl2, 20% Glycerol, pH7.5
Usage Method	冻干粉请按“复溶”说明用无菌去离子水复溶后使用，液体产品可直接使用；具体工作浓度请参考“推荐稀释比例”。
Target	CD73