Rabbit Anti-Human 44082 pAb
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产品编号
PA8636
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别名
SEPT8, 42980, SEP2, septin-8
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规格
- 50ul
- 100ul
Alias | SEPT8, 42980, SEP2, septin-8 |
Catalog Number | PA8636 |
Molecular Name | 44082 |
Host | Rabbit |
Size | 50ul, 100ul |
Clonity | Polyclonal |
Purification | Affinity Purification |
Formulation | Supply in PBS with 0.02% sodium azide, 50% glycerol, pH 7.4. |
Immunogen | Recombinant fusion protein containing a sequence corresponding to a.a. 348-427 of human SEPT8 (NP_001287728.1). |
Application | WB, IHC, IF |
Storage Condition | Store the product under sterile conditions after opening at -20℃. Avoid freeze-thaw cycles. |
Shipping Condition | Shipped on ice packs. |
Product Declaration | 本产品仅用于研究用途(RUO)。不能用于诊断或直接用于人体实验。 |
Product Full Name | Rabbit Anti-Human 44082 Polyclonal Antibody |
需确认编号对应蛋白(如转录因子、激酶等)。例如,若靶标为BRCA1,则参与DNA修复;若为PD-L1,则与免疫检查点调控相关。
①Western Blot:1:1000稀释,5%脱脂牛奶封闭,检测预期分子量条带(如BRCA1:220 kDa)。②免疫荧光(IF):1:200稀释,0.1% Triton X-100透膜,共聚焦显微镜观察亚细胞定位。
①使用靶标敲除(KO)细胞系作为阴性对照。②若靶标为磷酸化蛋白(如p-AKT),需验证经抑制剂处理的样本信号消失。
液体4℃保存6个月(含0.05% NaN₃),长期保存需分装-20℃(避免冻融)。
需测试与同家族蛋白的交叉反应(如AKT1/AKT2/AKT3)。