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Anti-Flag VHH, HRP

Anti-Flag VHH, HRP

产品编号

NAT101

别名

Anti Flag VHH, HRP, Anti-DYKDDDDK VHH, HRP, Anti DYKDDDDK VHH, HRP, Flag Tag, DYKDDDDK Tag, 抗Flag纳米抗体-HRP, 抗DYKDDDDK纳米抗体-HRP

规格
  • 50ug
  • 100ug
  • 200ug
产品介绍
Catalog NumberNAT101
AliasAnti Flag VHH, HRP, Anti-DYKDDDDK VHH, HRP, Anti DYKDDDDK VHH, HRP, Flag Tag, DYKDDDDK Tag, 抗Flag纳米抗体-HRP, 抗DYKDDDDK纳米抗体-HRP
Size50ug, 100ug, 200ug
Product DescriptionThe Anti-Flag VHH, HRP (NAT101) is a highly specific and affinity anti-Flag(DYKDDDDK) nano-antibody coupled with HRP, which can be used to detect the fusion protein containing Flag.
Molecular NameFlag
SpeciesAlpaca
HostYeast
ClonityMonoclonal
ConcentrationBatch to Batch
Purity>95% Determined by SDS-PAGE
PurificationAffinity purification
Storage ConditionStore at -20℃. Avoid repeated freezing and thawing cycles.
Temperature Range-20ºC
FormulationPBS, pH 7.4
Shipping ConditionShipped on ice packs.
BackgroundNano-antibody is a kind of variable region of heavy chain antibody which is found by scientists in 1989 and naturally lacks light chain. Its molecular weight is only 15 ku, which is 1/10 of that of conventional antibody. HRP-coupled Flag nano-antibody has small molecular weight (~15 KDa), strong specificity, high affinity and good stability, and can be produced stably with high quality, thus avoiding the difference between batches of traditional antibodies.
ImmunogenRecombinant Flag Protein
ApplicationELISA, WB
Recommended DilutionWB 1:5000-1:10000
TagHRP
Cross-reactivityNo cross-reactivity with other antigens or proteins.
Product DeclarationFor Research Use Only!
FAQ
  • 如何储存 Anti-Flag VHH, HRP 抗体?

    抗体可以在-20°C下储存以便常规使用,或-80°C用于长期保存。应分装储存并尽量减少冻融次数,以维持抗体的结合活性和稳定性。

  • 如何稀释 Anti-Flag VHH, HRP 抗体用于检测实验?

    建议使用PBS, pH 7.4缓冲液,并添加载体蛋白进行稀释。具体的稀释比例需根据实验类型(如Western Blot、ELISA)进行优化。稀释后的抗体建议在当天使用。为避免活性下降,请避免反复冻融原液,并尽量减少稀释后抗体在光下的暴露时间。

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