Anti-Flag VHH, HRP
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产品编号
NAT101
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别名
Anti Flag VHH, HRP, Anti-DYKDDDDK VHH, HRP, Anti DYKDDDDK VHH, HRP, Flag Tag, DYKDDDDK Tag, 抗Flag纳米抗体-HRP, 抗DYKDDDDK纳米抗体-HRP
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规格
- 50ug
- 100ug
- 200ug
| Catalog Number | NAT101 |
| Alias | Anti Flag VHH, HRP, Anti-DYKDDDDK VHH, HRP, Anti DYKDDDDK VHH, HRP, Flag Tag, DYKDDDDK Tag, 抗Flag纳米抗体-HRP, 抗DYKDDDDK纳米抗体-HRP |
| Size | 50ug, 100ug, 200ug |
| Product Description | The Anti-Flag VHH, HRP (NAT101) is a highly specific and affinity anti-Flag(DYKDDDDK) nano-antibody coupled with HRP, which can be used to detect the fusion protein containing Flag. |
| Molecular Name | Flag |
| Species | Alpaca |
| Host | Yeast |
| Clonity | Monoclonal |
| Concentration | Batch to Batch |
| Purity | >95% Determined by SDS-PAGE |
| Purification | Affinity purification |
| Storage Condition | Store at -20℃. Avoid repeated freezing and thawing cycles. |
| Temperature Range | -20ºC |
| Formulation | PBS, pH 7.4 |
| Shipping Condition | Shipped on ice packs. |
| Background | Nano-antibody is a kind of variable region of heavy chain antibody which is found by scientists in 1989 and naturally lacks light chain. Its molecular weight is only 15 ku, which is 1/10 of that of conventional antibody. HRP-coupled Flag nano-antibody has small molecular weight (~15 KDa), strong specificity, high affinity and good stability, and can be produced stably with high quality, thus avoiding the difference between batches of traditional antibodies. |
| Immunogen | Recombinant Flag Protein |
| Application | ELISA, WB |
| Recommended Dilution | WB 1:5000-1:10000 |
| Tag | HRP |
| Cross-reactivity | No cross-reactivity with other antigens or proteins. |
| Product Declaration | For Research Use Only! |
抗体可以在-20°C下储存以便常规使用,或-80°C用于长期保存。应分装储存并尽量减少冻融次数,以维持抗体的结合活性和稳定性。
建议使用PBS, pH 7.4缓冲液,并添加载体蛋白进行稀释。具体的稀释比例需根据实验类型(如Western Blot、ELISA)进行优化。稀释后的抗体建议在当天使用。为避免活性下降,请避免反复冻融原液,并尽量减少稀释后抗体在光下的暴露时间。
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