Mouse Anti-His Tag Monoclonal Antibody
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产品编号
MA200
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别名
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规格
- 100ul
- 500ul
Catalog Number | MA200 |
Species | Mouse |
Size | 100ul, 500ul |
Clonity | Monoclonal |
Protein Family | His Tag, Histidine |
Concentration | 1mg/ml |
Purification | Affinity purification |
Formulation | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Immunogen | A synthetic peptide of His tag |
Application | WB, IF, IP |
Recommended Dilution | WB 1:1000- 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:100 |
Storage Condition | Store at -20℃ for long term storage. Avoid repeated freezing and thawing cycles. |
Cross-reactivity | No cross-reactivity with other antigens or proteins. |
Product Declaration | 本产品仅用于研究用途(RUO)。不能用于诊断或直接用于人体实验。 |
适用于检测或纯化带有His标签(如6×His、10×His)的重组蛋白,常见应用包括Western Blot(WB)、免疫沉淀(IP)、免疫荧光(IF)及亲和层析纯化。
经测试无交叉反应,特异性针对多聚组氨酸标签(His Tag),不识别GST、FLAG、HA等常见标签或天然蛋白。
①稀释比例:WB建议1:1000-1:5000,IF/IP建议1:100-1:500(需根据样本优化)。②保存条件:分装后-20℃长期保存,避免反复冻融;短期可4℃保存(1个月内)。③样本处理:适用于变性(SDS-PAGE)或非变性(天然WB、IP)条件。