Rabbit Anti-Human AKT1 pAb
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产品编号
PA5305
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别名
AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, AKT1
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规格
- 50ul
- 100ul
Alias | AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, AKT1 |
Catalog Number | PA5305 |
Molecular Name | AKT1 |
Host | Rabbit |
Size | 50ul, 100ul |
Clonity | Polyclonal |
Purification | Affinity Purification |
Formulation | Supply in PBS with 0.02% sodium azide, 50% glycerol, pH 7.4. |
Immunogen | A synthetic peptide corresponding to a sequence within a.a. 400 to the C-terminus of human AKT1 (NP_005154.2). |
Application | WB, IHC, IF, IP |
Storage Condition | Store the product under sterile conditions after opening at -20℃. Avoid freeze-thaw cycles. |
Shipping Condition | Shipped on ice packs. |
Product Declaration | 本产品仅用于研究用途(RUO)。不能用于诊断或直接用于人体实验。 |
Product Full Name | Rabbit Anti-Human AKT1 Polyclonal Antibody |
AKT1主要调控细胞存活和增殖,而AKT2侧重代谢调控;该抗体特异性针对AKT1,与AKT2/AKT3无交叉反应。
不能,此抗体检测总AKT1蛋白,需配合磷酸化特异性抗体(如p-AKT1 Ser473)分析激活状态。
适用于乳腺癌、胶质瘤等AKT1高表达肿瘤,建议使用PTEN缺失细胞系(如U87MG)作为阳性对照。
使用温和裂解液(如NP-40),避免剧烈震荡,并预清除样本以减少非特异性结合。
采用高压热修复(pH 9.0 EDTA缓冲液),并延长一抗孵育时间(4℃过夜)。