Rabbit Anti-AGO3 Polyclonal Antibody
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产品编号
PAV344
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别名
argonaute RISC catalytic component 3, EIF2C3
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规格
- 100ul
Alias | argonaute RISC catalytic component 3, EIF2C3 |
Catalog Number | PAV344 |
Molecular Name | AGO3 |
Host | Rabbit |
Size | 100ul |
Clonity | Polyclonal |
Concentration | 1 mg/ml |
Purification | affinity-chromatography |
Formulation | supply in PBS containing 50% glycerol, and 0.02% sodium azide. |
Immunogen | This antibody was raised against from part region of human protein |
Recommended Dilution | WB 1:500-2000 ELISA 1:5000-20000 |
Storage Condition | Store at 4℃ for for short term storage. It is recommended to aliquote to ~10ul per vial and store at -20℃ for long term storage. Avoid repeated freezing and thawing cycles. |
Shipping Condition | This product is shipped on blue ice. |
Product Declaration | 本产品仅用于研究用途(RUO)。不能用于诊断或直接用于人体实验。 |
AGO3参与piRNA通路及转座子沉默,与AGO2(miRNA/siRNA通路)在RNA沉默机制中分工不同。
主要识别人源AGO3,与果蝇/小鼠AGO3同源性较低,需预实验验证。
结合RNA免疫沉淀(RIP)及piRNA测序,验证AGO3结合的piRNA分子。
预测分子量约87 kDa,需通过阳性对照(如睾丸组织)验证。
可以,推荐使用睾丸或卵巢组织,优化透膜条件(0.5% Triton X-10分钟)。