Streptococcus Pyogenes CRISPR-Cas9 Protein, His Tag
-
产品编号
KMP4412
-
别名
-
规格
- 50ug
- 100ug
- 200ug
产品介绍
| Catalog Number | KMP4412 |
| Molecular Name | CRISPR-Cas9 |
| Size | 50ug, 100ug, 200ug |
| Purity | >90% as determined by SDS-PAGE |
| Product Description | The Streptococcus Pyogenes CRISPR-Cas9 Protein, His Tag(KMP4412) is produced in Insect Cells. A DNA sequence encoding the s. pyogenes serotype M1 cas9 (WP_010922251.1) (Met1-Asp1368) was expressed with a nuclear localization sequence (NLS) at the N-terminus, two NLSs and a polyhistidine tag at the C-terminus. |
| Storage Condition | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
| Formulation | 20mM Tris, 300mM NaCl, 50% Glycerol, 0.5mM PMSF, 0.1mM EDTA, 0.5mM TCEP, pH8.0 |
| Shipping Condition | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice. |
| Species | Streptococcus Pyogenes |
| Host | Insect Cells |
| Purification | Affinity purification |
| Endotoxin | <1.0 EU/ug determined by the LAL method |
FAQ
-
Q1-该蛋白是否具有核酸酶活性？
是的，Cas9蛋白在sgRNA引导下可靶向切割DNA。
-
Q2-能否用于基因编辑实验？
可以，广泛应用于体外或细胞内的基因敲除、敲入等研究。
-
Q3-His标签是否影响功能？
不影响，标签位于蛋白末端，经功能验证活性完好。
-
Q4-是否需要额外添加RNase抑制剂？
建议在实验体系中加入RNase抑制剂以保护sgRNA。
-
Q5-是否有预复合sgRNA的成品提供？
支持定制Cas9-sgRNA复合体，需提前联系确认序列。
- 联系我们
- 电话：400-621-6806
- 手机：13116038708
- 邮箱：info@kmdbioscience.com
- 地址：天津市武清区国际企业社区A5栋
-
销售微信
-
官方公众号
-
400-621-6806 -
614353794 -
周一至周五 8:30-18:00 -
-
0
-
