E. coli LON PROTEASE Protein, His Tag_天津卡梅德科技发展有限公司

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E. coli LON PROTEASE Protein, His Tag

E. coli LON PROTEASE Protein, His Tag

产品编号

KMP4411

别名

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Catalog Number	KMP4411
Molecular Name	LON PROTEASE
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Product Description	The E. coli LON PROTEASE Protein, His Tag(KMP4411) is produced in E. coli. A DNA sequence encoding E.coil Lon (P0A9M0) (Met1-Lys784) was expressed with a polyhistidine tag at the C-terminus.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	PBS, 10% Glycerol, pH7.4
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.
Background	Lon protease, an ATP-dependent mitochondrial protease, is important in mitochondrial protein maintenance. Lon protease is a multifunctional enzyme, and its functions include the degradation of damaged proteins and naturally short-lived proteins, ATPase and chaperone-like activities, as well as DNA binding. Lon protease plays a major role in the protein quality control system in mammalian cell mitochondria. It is present in the mitochondrial matrix and degrades oxidized and misfolded proteins, thereby protecting the cell from various extracellular stresses, including oxidative stress. The intellectual disability-associated and thalidomide-binding protein cereblon (CRBN) contains a large, highly conserved Lon domain. The Lon ATP-dependent protease plays an important role in regulating many biological processes in bacteria.
Species	E. coli
Host	E. coli
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method

FAQ

Q1-Lon蛋白酶的主要功能是什么？

Lon蛋白酶是一种ATP依赖的蛋白酶，负责降解错误折叠蛋白和调控应激反应，维持细菌内环境稳态。
Q2-该蛋白是否具有ATP酶活性？

是的，重组Lon蛋白酶经检测具有ATP水解活性，可通过荧光法（如Malachite Green法）定量分析。
Q3-能否用于体外蛋白降解实验？

适用于研究特定底物（如SulA）的降解动力学，需补充ATP和Mg²⁺以激活酶活性。
Q4-His标签是否干扰底物识别？

无干扰，实验验证显示带His标签的Lon蛋白酶底物结合能力与天然酶一致。
Q5-储存缓冲液中为何添加DTT？

DTT（二硫苏糖醇）用于维持酶的还原状态，防止氧化导致的活性丧失，建议使用前勿移除。

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