Rabbit Anti-TOP1M/TOP1MT Polyclonal Antibody
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产品编号
PAV4095
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别名
DNA topoisomerase I mitochondrial
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目录价格
InquiryRMB
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规格
- 100ul
产品介绍
| Alias | DNA topoisomerase I mitochondrial |
| Catalog Number | PAV4095 |
| Product Description | Rabbit polyclonal antibody raised against human TOP1MT (DNA topoisomerase I, mitochondrial). Affinity purified from rabbit antiserum by antigen-specific affinity chromatography. Validated applications: WB, ELISA. Supplied as liquid in PBS containing 50% glycerol and 0.02% sodium azide at 1 mg/ml. |
| Molecular Name | TOP1MT |
| Species | Human, Mouse, Rat |
| Host | Rabbit |
| Size | 100ul |
| Clonity | Polyclonal |
| Protein Family | 线粒体蛋白 |
| Concentration | 1 mg/ml |
| Purification | affinity-chromatography |
| Formulation | supply in PBS containing 50% glycerol, and 0.02% sodium azide. |
| Background | This gene encodes a mitochondrial DNA topoisomerase that plays a role in the modification of DNA topology. The encoded protein is a type IB topoisomerase and catalyzes the transient breaking and rejoining of DNA to relieve tension and DNA supercoiling generated in the mitochondrial genome during replication and transcription. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012] |
| Immunogen | This antibody was raised against from part region of human TOP1MT protein (UniProt: Q969P6) |
| Application | WB, ELISA |
| Recommended Dilution | WB 1:500-2000 ELISA 1:5000-20000 |
| Gene ID | 116447 |
| Tag | Unconjugated |
| Cross-reactivity | Human, Mouse, Rat |
| Predicted Molecular Weight | ≈69.9 kDa |
| Storage Condition | Store at 4℃ for for short term storage. It is recommended to aliquote to ~10ul per vial and store at -20℃ for long term storage. Avoid repeated freezing and thawing cycles. |
| Shipping Condition | This product is shipped on blue ice. |
| Molecular Weight | 69.9 kDa |
| Cross-reactivity | Human, Mouse, Rat |
| Uniprot ID | Q969P6 |
| Note | Gene ID: 116447; UniProt: Q969P6; Chromosome: 8q24.3; Predicted MW: 69.9 kDa |
| Function | Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). |
| Product Declaration | 本产品仅用于研究用途(RUO)。不能用于诊断或直接用于人体实验。 |
| Product Full Name | Rabbit Anti-Human TOP1MT Polyclonal Antibody |
| Target | TOP1MT |
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